Wingless, decapentaplegic and EGF receptor signaling pathways interact to specify dorso-ventral pattern in the adult abdomen of Drosophila.
Wingless, decapentaplegic and EGF receptor signaling pathways interact to specify dorso-ventral pattern in the adult abdomen of Drosophila.
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发表时间：
1999-08
期刊：
影响因子：
4.6
通讯作者：
Artyom Kopp;Ronald K. Blackman;Ian J. Duncan
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作者：
Artyom Kopp;Ronald K. Blackman;Ian J. Duncan
果蝇成虫腹节沿背腹轴沿着分为背侧片、腹侧片和腹侧片。我们报告说，这种模式在很大程度上是指定在蛹阶段的无翅（Wg），十肢麻痹（Dpp）和果蝇EGF受体（DER）信号。通过Hedgehog信号在前室的后缘激活wg和dpp的表达。在该区域内，wg和dpp表达在沿着背腹轴相互排斥的域中：wg表达在胸骨和中-外侧端，而dpp表达仅限于胸膜和背中线。这两个基因都不表达在外侧termium。Shirras和Couso（1996，Dev. 175，24-36）已经表明，term和胸骨细胞命运由Wg信号传导指定。我们发现DER与Wg协同作用，促进背板和胸骨的身份，并且Wg和DER活动受到Dpp信号传导的反对，从而促进胸膜的身份。Wg和Dpp在两个层面上相互对抗。首先，它们的表达被限制在互补结构域的相互转录抑制。第二，Wg和Dpp通过对细胞命运施加相反的影响而彼此直接竞争。DER信号不影响wg或dpp的表达，表明其在细胞命运决定水平上与Wg和Dpp相互作用。在term中，对Wg和DER功能的要求大致互补：Wg主要在内侧区域需要，而DER在外侧区域最重要。最后，我们表明，Dpp信号在背中线控制背腹图案内tertertermos通过促进色素沉着的内侧区域。
Adult abdominal segments of Drosophila are subdivided along the dorso-ventral axis into a dorsal tergite, a ventral sternite and ventro-lateral pleural cuticle. We report that this pattern is largely specified during the pupal stage by Wingless (Wg), Decapentaplegic (Dpp) and Drosophila EGF Receptor (DER) signaling. Expression of wg and dpp is activated at the posterior edge of the anterior compartment by Hedgehog signaling. Within this region, wg and dpp are expressed in domains that are mutually exclusive along the dorso-ventral axis: wg is expressed in the sternite and medio-lateral tergite, whereas dpp expression is confined to the pleura and the dorsal midline. Neither gene is expressed in the lateral tergite. Shirras and Couso (1996, Dev. Biol. 175, 24-36) have shown that tergite and sternite cell fates are specified by Wg signaling. We find that DER acts synergistically with Wg to promote tergite and sternite identities, and that Wg and DER activities are opposed by Dpp signaling, which promotes pleural identity. Wg and Dpp interact antagonistically at two levels. First, their expression is confined to complementary domains by mutual transcriptional repression. Second, Wg and Dpp compete directly with one another by exerting opposite effects on cell fate. DER signaling does not affect the expression of wg or dpp, indicating that it interacts with Wg and Dpp at the level of cell fate determination. Within the tergite, the requirements for Wg and DER function are roughly complementary: Wg is required mainly in the medial region, whereas DER is most important laterally. Finally, we show that Dpp signaling at the dorsal midline controls dorso-ventral patterning within the tergite by promoting pigmentation in the medial region.