Primary structure of the replication initiation protein of plasmid R6K.
Primary structure of the replication initiation protein of plasmid R6K.
复制标题
质粒 R6K 复制起始蛋白的一级结构。
DOI：
10.1073/pnas.79.18.5475
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发表时间：
1982
影响因子：
11.1
通讯作者：
Bastia,D
中科院分区：
文献类型：
--
作者：
Germino,J;Bastia,D
将质粒R6 K的复制起始蛋白的顺反子克隆到单链DNA载体M13 mp8和M13 mp 9中，并测定了其核苷酸全序列。从其核苷酸序列预测的起始蛋白的氨基酸序列显示，该蛋白富含赖氨酸且呈弱碱性，分子量为35，000，这与从NaDodSO 4/丙烯酰胺凝胶中的迁移率估计的分子量非常一致。蛋白质的二级结构，大约通过Chou和Fasman的概率方法[Chou，P. & Fasman，G.（1978）Adv. Enzymol. 47，45-148]，提出了主要带正电荷的α-螺旋结构的NH 2-末端结构域，散布的无规卷曲和β-折叠和β-转角的短延伸的核心区域，以及α-螺旋的COOH-末端结构域。
The cistron of the replication initiation protein of plasmid R6K has been cloned into the single-strand DNA vectors M13mp8 and M13mp9 and its complete nucleotide sequence has been determined. The amino acid sequence of the initiator protein as predicted from its nucleotide sequence shows that the protein is lysine rich and weakly basic and has a molecular weight of 35,000, which is in close agreement with that estimated from the mobility in NaDodSO4/acrylamide gels. The secondary structure of the protein, approximately by the probabilistic methods of Chou and Fasman [Chou, P. & Fasman, G. (1978) Adv. Enzymol. 47, 45-148], suggests an NH2-terminal domain of primarily positively charged alpha-helical structure, a core region of interspersed short stretches of random coils and beta-sheets and -turns, and a COOH-terminal domain of alpha-helix.