Quantum dot-aptamer nanoprobes for recognizing and labeling influenza A virus particles
Quantum dot-aptamer nanoprobes for recognizing and labeling influenza A virus particles
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DOI：
10.1039/c1nr10218d
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发表时间：
2011-01-01
期刊：
影响因子：
6.7
通讯作者：
Zhang, Xian-En
中科院分区：
文献类型：
--
作者：
Cui, Zong-Qiang;Ren, Qian;Zhang, Xian-En
病毒的荧光标记是一种有用的病毒检测和成像技术。结合量子点优良的荧光特性和适体的高亲和力和特异性，构建了量子点适体探针。将抗甲型流感病毒血凝素的适体A22与量子点连接，产生QD-A22探针。荧光成像和透射电镜显示，QD-A22探针能特异性识别和标记甲型流感病毒颗粒。这种QD标记技术为病毒检测和成像提供了一种新的病毒颗粒标记策略。
The fluorescence labeling of viruses is a useful technology for virus detection and imaging. By combining the excellent fluorescence properties of quantum dots (QDs) with the high affinity and specificity of aptamers, we constructed a QD-aptamer probe. The aptamer A22, against the hemagglutinin of influenza A virus, was linked to QDs, producing the QD-A22 probe. Fluorescence imaging and transmission electron microscopy showed that the QD-A22 probe could specifically recognize and label influenza A virus particles. This QD labeling technique provides a new strategy for labeling virus particles for virus detection and imaging.