Anti-inflammatory effects of south American Tanacetum vulgare
Anti-inflammatory effects of south American Tanacetum vulgare
复制标题
DOI：
10.1111/j.2042-7158.1998.tb06924.x
复制
发表时间：
1998-09-01
影响因子：
3.3
通讯作者：
Máñez, S
中科院分区：
文献类型：
--
作者：
Schinella, GR;Giner, RM;Máñez, S
近年来，酚类化合物和倍半萜内酯，尤其是小白菊内酯，在菊科植物菊科植物中的抗偏头痛和抗炎作用引起了广泛关注。然而，密切相关的世界性物种 T. vulgare 仍处于该领域研究的主流之外。用二氯甲烷和甲醇处理 T. vulgare 的地上部分，并应用常规柱和薄层色谱技术，可以从中等亲脂性部分中分离出该植物对小鼠耳的抗炎活性的原理。 12-O-十四烷酰佛波醇 13-乙酸酯诱导的水肿。通过紫外和核磁共振光谱鉴定，这些药物为小白菊内酯（0.5 mg/耳，水肿抑制 93%，ID50（抑制水肿的药物剂量）= 0.18 μ mol/耳）和甲氧基黄酮 jaceosidin（0.5 mg/耳，水肿抑制 80%，ID50 = 0.50 μ mol/耳）、紫茎泽兰、 因为以摩尔计算，小白菊内酯的效力几乎是最活跃的黄酮的三倍，并且因为它是从植物中获得的，数量相当多，所以黄酮类化合物必须仅对观察到的体内抗炎局部作用负有部分责任，并且在较小程度上负责。
In recent years the role of phenolic compounds and sesquiterpene lactones, particularly parthpnolide, in the anti-migraine and anti-inflammatory effects of Tanacetum parthenium (Asteraceae) has attracted much attention. However, the closely-related cosmopolitan species T. vulgare has remained outside the mainstream of research in this field.After treating the aerial parts of T. vulgare with dichloromethane and methanol, and applying conventional column and thin-layer chromatographic techniques, it was possible to isolate from the moderately lipophilic fractions the principles responsible for the antiinflammatory activity of this plant against the mouse-ear oedema induced by 12-O-tetradecanoylphorbol 13-acetate. These were identified by ultraviolet and nuclear magnetic resonance spectroscopy as parthenolide (93% oedema inhibition at 0.5 mg/ear, ID50 (dose of drug inhibiting the oedema by 50%) = 0.18 mu mol/ear) and the methoxyflavones jaceosidin (80% oedema inhibition at 0.5 mg/ear, ID50 = 0.50 mu mol/ear), eupatorin, chrysoeriol and diosmetin.Because in molar terms the potency of parthenolide was nearly three times greater than that of the most active of the flavones and because it is obtained from the plant in considerably larger amounts, the flavonoids must only be partially responsible, and to a minor extent, for the observed in-vivo anti-inflammatory local effect.