Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro.
Prostaglandin E2 promotes human CD34+ cells homing through EP2 and EP4 in vitro.
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前列腺素 E2 在体外通过 EP2 和 EP4 促进人 CD34( ) 细胞归巢
DOI：
10.3892/mmr.2017.6649
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发表时间：
2017-07
影响因子：
3.4
通讯作者：
Xu D
中科院分区：
文献类型：
--
作者：
Wang Y;Lai S;Tang J;Feng C;Liu F;Su C;Zou W;Chen H;Xu D
最近，一些研究在体外证明前列腺素E2(PGE2)促进人类分化簇(CD)34+细胞归巢。然而，被PGE2激活的亚型受体尚不清楚，因为PGE2受体EP1-4亚型(EP1-4)表达在人CD34+细胞膜上。基于此，本研究旨在筛选PGE2促进人CD34+细胞归巢的受体亚型活性。观察到人CD34+细胞表达4种PGE2亚受体，尤其是EP2和EP4。PGE2显著增加EP2和4mRNA的表达，而EP1和3mRNA的表达无明显变化。前列腺素E_2、EP2激动剂和EP4A上调人CD34+细胞C-X-C趋化因子受体4mRNA和蛋白的表达，促进骨髓间充质干细胞基质细胞衍生因子1α(SDF-1α)的表达。这些现象可被相关的受体拮抗剂抑制。PGE2、EP2a和EP4A促进人CD34+细胞向SDF-1α和BMMSCs迁移。本研究结果提示，PGE2可通过EP2和EP4受体促进人CD34+细胞归巢。
Recently, certain studies have demonstrated in vitro that prostaglandin E2 (PGE2) promotes human cluster of differentiation (CD)34+ cell homing. However, the sub-type receptors activated by PGE2 are unknown, as the PGE2 receptor EP1-4 subtypes (EP1-4) are expressed on the membrane of human CD34+ cells. Based on the above, the present study aimed to screen the receptor subtype activity by PGE2 to promote human CD34+ cell homing. It was observed that human CD34+ cells expressed the four PGE2 sub-receptors, particularly EP2 and 4. PGE2 increased EP2 and 4 mRNA expression significantly, while EP1 and 3 mRNA exhibited no significant alteration. PGE2, EP2 agonist (EP2A), and EP4A upregulated C-X-C chemokine receptor 4 mRNA and protein expression in human CD34+ cells, and promoted stromal cell-derived factor 1α (SDF-1α) expression in bone marrow mesenchymal stem cells (BMMSCs). These phenomena were inhibited by the associated receptor antagonists. PGE2, EP2A, and EP4A facilitated human CD34+ cell migration towards SDF-1α and BMMSCs. The results of the present study suggested that PGE2 promoted human CD34+ cell homing through EP2 and 4 receptors in vitro.