Phylogenetic Relationship in Different Commercial Strains of Pleurotus nebrodensis Based on ITS Sequence and RAPD.
Phylogenetic Relationship in Different Commercial Strains of Pleurotus nebrodensis Based on ITS Sequence and RAPD.
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DOI：
10.4489/myco.2009.37.3.183
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发表时间：
2009-09
期刊：
影响因子：
1.9
通讯作者：
Lee TS
中科院分区：
文献类型：
--
作者：
Alam N;Shim MJ;Lee MW;Shin PG;Yoo YB;Lee TS
利用内转录间隔区（ITS）和RAPD对9个不同商业栽培侧耳菌的分子系统发育进行了研究。所选菌株ITS区序列显示，ITS区总长度在592 ~ 614 bp之间。各菌株ITS1和ITS2区大小分别在219 ~ 228 bp和211 ~ 229 bp之间。ITS2序列比ITS1变化更大，5.8S序列区域相同。ITS区序列的系统进化树显示，所选菌株可分为5个聚类。ITS区域序列的互反同源性在99 ~ 100%之间。用任意20条引物对菌株进行RAPD分析。12条引物可有效扩增基因组DNA。获得的多态片段大小在200 ~ 2000 bp之间。RAPD和ITS分析技术能够检测到被试菌株之间的遗传变异。实验结果表明，IUM-1381、IUM-3914、IUM-1495和AY-581431菌株的遗传相似性非常高。因此，所有的IUM和NCBI基因库菌株在遗传上是相同的，但存在一定的变异。
The molecular phylogeny in nine different commercial cultivated strains of Pleurotus nebrodensis was studied based on their internal transcribed spacer (ITS) region and RAPD. In the sequence of ITS region of selected strains, it was revealed that the total length ranged from 592 to 614 bp. The size of ITS1 and ITS2 regions varied among the strains from 219 to 228 bp and 211 to 229 bp, respectively. The sequence of ITS2 was more variable than ITS1 and the region of 5.8S sequences were identical. Phylogenetic tree of the ITS region sequences indicated that selected strains were classified into five clusters. The reciprocal homologies of the ITS region sequences ranged from 99 to 100%. The strains were also analyzed by RAPD with 20 arbitrary primers. Twelve primers were efficient to applying amplification of the genomic DNA. The sizes of the polymorphic fragments obtained were in the range of 200 to 2000 bp. RAPD and ITS analysis techniques were able to detect genetic variation among the tested strains. Experimental results suggested that IUM-1381, IUM-3914, IUM-1495 and AY-581431 strains were genetically very similar. Therefore, all IUM and NCBI gene bank strains of P. nebrodensis were genetically same with some variations.