Morphometric and Stereologic Analysis of Chlorella vulgaris Under Heterotrophic Growth Conditions
Morphometric and Stereologic Analysis of Chlorella vulgaris Under Heterotrophic Growth Conditions
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DOI：
10.1093/oxfordjournals.aob.a088128
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发表时间：
1991-03
期刊：
影响因子：
4.2
通讯作者：
F. Martínez;C. Ascaso;M. Orús
中科院分区：
文献类型：
--
作者：
F. Martínez;C. Ascaso;M. Orús
利用计算机辅助体视学参数测定技术研究了小球藻UAM 101在异养、兼养和光合异养条件下超微结构的变化。数据记录是通过半自动数字化图像分析系统进行的，而不是目前的短线阵列叠加方法。葡萄糖促进了剧烈的生理变化[Martinez和Orus. 1991.植物生理学（出版中）]，这强烈影响了存储材料的细胞大小和体积密度。然而，葡萄糖的存在下，可能表明这些比率是每个物种的特征的叶绿体或叶绿体活性部分的体积比没有受到影响。细胞壁超微结构也进行了分析，并证明了孢粉素的存在下，与薄和缺乏孢粉素的细胞壁一般描述的小球藻物种。
A computer-supported determination of stereological paramelers was used to study the possible ultrastructural changes of Chlorella vulgairis UAM 101 under photolithotrophic, mixotrophic and photoheterotrophic conditions of growth. Data recording was carried out through a semi-automatic digitizing image analysis system instead of the current method of superimposition of an array of short lines. Glucose promoted drastic physiological changes [Martinez and Orus. 1991. Plant Physiology (in press)], which strongly affected the size of the cells and volume densities of storage materials. However, volumetric ratios of the mitochondrion or chloroplast active fraction were not affected by the presence of glucose, probably indicating that these ratios are characteristic of each species. Cell wall ultrastructure was also analysed and the presence of sporopollenin demonstrated, in contrast to the thin and sporopollenin-lacking cell wall generally described for Chlorella vulgaris species.