Probiotic Lactobacilli Interfere with Streptococcus mutans Biofilm Formation In Vitro
Probiotic Lactobacilli Interfere with Streptococcus mutans Biofilm Formation In Vitro
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DOI：
10.1007/s00284-010-9752-9
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发表时间：
2011-02-01
影响因子：
2.6
通讯作者：
Haukioja, Anna L.
中科院分区：
文献类型：
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作者：
Soderling, Eva M.;Marttinen, Aino M.;Haukioja, Anna L.
在临床研究中，益生菌减少了唾液中变形链球菌（MS）的数量。我们比较了益生乳酸菌菌株对变形链球菌生物膜形成的影响。所用菌株包括4株S.变形杆菌菌株（参考菌株NCTC 10449和Ingbritt以及临床分离株2366和195）和益生菌菌株鼠李糖乳杆菌GG，L. plantarum 299v和L.罗伊氏菌株PTA 5289和SD 2112。在乳酸杆菌（LB）存在下，研究了MS在玻璃表面上粘附和生长的能力，反映了生物膜的形成。还研究了LB培养上清液对MS活力的影响。LB对MS临床分离株的生物被膜形成均有抑制作用（P <0.001）。LB对MS参比菌株的生物膜形成也有抑制作用，但对L.植物L. reuteri PTA 5289的抑制作用弱于L. reuteri SD 2112和L.鼠李糖可行S.只有当用L. reuteri菌株。洛杉矶在用培养物上清液进行的试验中，罗伊氏菌株杀死MS的效率也较低。L. reuteri比L. rhamnosus GG和L.跖侧; P <0.001。总之，我们的研究结果表明，四种常用的益生菌干扰S。体外对变形链球菌生物膜形成的影响，以及对变形链球菌的抗菌活性。变形杆菌是pH依赖性的。
In clinical studies, probiotic bacteria have decreased the counts of salivary mutans streptococci (MS). We compared the effects of probiotic Lactobacillus strains on the biofilm formation of Streptococcus mutans. The bacterial strains used included four S. mutans strains (reference strains NCTC 10449 and Ingbritt and clinical isolates 2366 and 195) and probiotic strains Lactobacillus rhamnosus GG, L. plantarum 299v, and L. reuteri strains PTA 5289 and SD2112. The ability of MS to adhere and grow on a glass surface, reflecting biofilm formation, was studied in the presence of the lactobacilli (LB). The effect of LB culture supernatants on the viability of the MS was studied as well. All of the LB inhibited the biofilm formation of the clinical isolates of MS (P < 0.001). The biofilm formation of the reference strains of MS was also inhibited by the LB, but L. plantarum and L. reuteri PTA 5289 showed a weaker inhibition when compared to L. reuteri SD2112 and L. rhamnosus GG. Viable S. mutans cells could be detected in the biofilms and culture media only when the experiments were performed with the L. reuteri strains. The L. reuteri strains were less efficient in killing the MS also in the tests performed with the culture supernatants. The pHs of the supernatants of L. reuteri were higher compared to those of L. rhamnosus GG and L. plantarum; P < 0.001. In conclusion, our results demonstrated that four commonly used probiotics interfered with S. mutans biofilm formation in vitro, and that the antimicrobial activity against S. mutans was pH-dependent.