Field test results using the BioPEPR cervical smear prescreening system.
Field test results using the BioPEPR cervical smear prescreening system.
复制标题
使用 BioPEPR 宫颈涂片预筛查系统的现场测试结果。
DOI：
10.1002/cyto.990010305
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
R. Walle
中科院分区：
文献类型：
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作者：
Zahniser Dj;P. Oud;Raaijmakers Mc;G. P. Vooys;R. Walle
BioPEPR是一种阴极射线管扫描设备，已开发用于宫颈涂片的预筛查。宫颈样本在悬浮液中收集，注射，计数并铺在显微镜载玻片上。用刚果红的feulgen - thiionine作为染色程序。使用中间照相步骤，以1 μ分辨率扫描6 X 8 mm的区域。根据分层决策策略的要求，测量细胞的形态参数。目前每次涂片的分析速度约为4分钟；预计至少会有四倍的改进。目前正在与当地人口筛查项目合作，对BioPEPR进行实地试验。在这项研究中，每位女性都做了两次涂片；前者根据Papanicolaou技术制备，后者根据BioPEPR方法制备并染色。除了人口筛查项目的涂片外，还从几个妇科诊所获得了一些可能异常的涂片。迄今为止，BioPEPR系统已经分析了大约3500张“秒”涂片。结果显示漏报率约为几个百分点（没有漏报比轻微的不典型增生更严重），误报率约为24%。进一步分析虚警率的年龄分布表明，很大一部分虚警来自50岁以上的女性；50岁以下女性的误报率约为20%。
BioPEPR is a cathode ray tube scanning device that has been developed for the prescreening of cervical smears. Cervical samples are collected in suspension, syringed, counted and spread across a microscope slide. Feulgen-Thionine with Congo Red is used as the staining procedure. Fields of 6 X 8 mm are scanned at 1 mu resolution, using an intermediate photographic step. Morphologic parameters of the cells are measured, as called for by a hierarchical decision strategy. Analysis speed is currently about 4 min per smear; improvements of at least a factor of four are expected. A field test of BioPEPR is being undertaken in cooperation with a local population screening program. In the study two smears are made from each woman; the first is prepared according to the Papanicolaou technique while the second is prepared and stained according to the BioPEPR methods. In addition to smears from the population screening program a number of possibly abnormal smears are obtained from several gynecological clinics. To date approximately 3500 "second" smears have been analyzed by the BioPEPR system. The results show a missed positive rate on the order of a few percent (no missed positives were more severe than a slight dysplasia), and a false alarm rate of about 24%. A further analysis of the age distribution of the false alarm rate shows that a large proportion came from women above the age of 50; the false alarm rate is about 20% for women under the age of 50.