Antibody to Reduction Modifiable Protein Increases the Bacterial Burden and the Duration of Gonococcal Infection in a Mouse Model
Antibody to Reduction Modifiable Protein Increases the Bacterial Burden and the Duration of Gonococcal Infection in a Mouse Model
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DOI：
10.1093/infdis/jiv024
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发表时间：
2015-07-15
影响因子：
6.4
通讯作者：
Rice, Peter A.
中科院分区：
文献类型：
--
作者：
Gulati, Sunita;Mu, Xin;Rice, Peter A.
抗还原修饰蛋白抗体（抗rmp抗体）可以阻断其他抗菌抗体对淋病奈瑟菌的补体依赖性杀伤。一种抗低脂多糖抗菌单克隆抗体（mAb） 2C7，一种淋球菌候选疫苗，可减少淋球菌在BALB/c小鼠阴道定毒。在这里，我们发现抗rmp抗体以剂量依赖的方式阻断小鼠单抗2C7的功效。抗rmp抗体也能在体内对抗2c7介导的C3在淋球菌上沉积的增强。该小鼠模型将有助于研究阻断抗体如何影响淋球菌疫苗的效力。预先存在的抗rmp抗体将是评估淋球菌候选疫苗有效性的重要考虑因素。
Antibodies against reduction modifiable protein (anti-Rmp Abs) can block complement-dependent killing of Neisseria gonorrhoeae by otherwise bactericidal Abs. An anti-lipooligosaccharide bactericidal monoclonal Ab (mAb) 2C7, a gonococcal vaccine candidate Ab, attenuates vaginal colonization by gonococci in BALB/c mice. Here we show that anti-Rmp Abs block the efficacy of mAb 2C7 in mice in a dose-dependent manner. Anti-Rmp Abs also counteract 2C7-mediated enhancement of C3 deposition on gonococci in vivo. The mouse model will prove useful to study how blocking Abs influence the efficacy of gonococcal vaccines. Preexisting anti-Rmp Abs will be an important consideration in evaluating the efficacy of gonococcal vaccine candidates.