The specific amino acid requirements of a mammalian cell (strain L) in tissue culture.
The specific amino acid requirements of a mammalian cell (strain L) in tissue culture.
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组织培养中哺乳动物细胞（L 株）的特定氨基酸需求。
DOI：
10.1016/s0021-9258(18)70932-0
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发表时间：
1955
期刊：
影响因子：
--
通讯作者：
H. Eagle
中科院分区：
文献类型：
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作者：
H. Eagle
这些细胞是L品系的小鼠成纤维细胞，生长在玻璃表面上的单细胞层中。基础培养基含有19种氨基酸、已知维生素、无机盐、胆碱、葡萄糖、谷氨酰胺、谷胱甘肽和肌醇，并补充0.25%至2%的透析马血清。在这种培养基中，培养物可以保持8周之久，而细胞的生长速度或外观没有变化，通过依次从培养基中省略它们，表明精氨酸、胱氨酸、组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、色氨酸、酪氨酸和缬氨酸是生存和生长所必需的。丙氨酸、天冬氨酸、谷氨酸、甘氨酸、羟脯氨酸、脯氨酸和丝氨酸不是必需的;不太可能是培养基中的少量血清蛋白提供的。任何一种必需氨基酸缺乏的细胞学效应在2 ~ 6天内是可逆的。最大生长所需的必需氨基酸浓度从每毫升0.005微摩尔色氨酸不等。至0.1或0.2 μ mol异亮氨酸/ml，低于这些浓度，生长率急剧下降。12种必需的L-氨基酸中没有一种可以被D-异构体取代，但是D-异构体并不积极地抑制生长。尽管培养物需要12种氨基酸，但是精氨酸、胱氨酸和酪氨酸对于小鼠来说不是必需的。D.邓肯。
The cells were of the L strain of mouse fibroblast, grown in a monocellular layer on a glass surface. The basal medium contained 19 aminoacids, the known vitamins, inorganic salts, choline, glucose, glutamine, glutathione and inositol and was supplemented with 0.25 to 2 per cent. of dialysed horse serum. In this medium cultures could be maintained for as much as 8 weeks without change in growth rate or appearance of the cells.By omitting them from the medium in turn it was shown that arginine, cystine, histidine,isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, tyrosine and valine were essential for survival and growth. Alanine, aspartic acid, glutamic acid, glycine, hydroxyproline, proline and serine were not essential; it is unlikely that the small amount of serum protein in the medium was supplying them. The cytological effects of deficiency of any one essential aminoacid were reversible for 2 to 6 days. The concentrations of essential amino-acids needed for maximum growth varied from 0.005 µmole tryptophan per ml. to 0.1 or 0.2 µmoleisoleucineper ml., and below these concentrations the rate of growth fell sharply. None of the 12 essential L-aminoacids could be replaced by the D-isomer, but the D-isomers did not actively inhibit growth.Although the cultures required 12 amino-acids, arginine, cystine and tyrosine are not essential for the mouse.-D. Duncan.