In vitro cultivation of epithelial cells derived from tumors of the human urinary tract.
In vitro cultivation of epithelial cells derived from tumors of the human urinary tract.
复制标题
体外培养源自人类泌尿道肿瘤的上皮细胞。
作者：
Elliott Ay;David L. Bronson;N. Stein;Elwin E. Fraley
将来自54个人尿路TCC 2的切碎的外植体体外培养，试图建立细胞系。从48个肿瘤外植体中生长出具有上皮形态的细胞，并从10个中建立长期细胞培养物。其中6个细胞培养物已维持超过18个月，有50 - 70次转移，因此被认为是永久性细胞系。已建立的培养物中的上皮细胞较小，倍增时间快，且呈多层结构。通过显微镜和培养技术检查细胞，发现其不含污染微生物，包括支原体。已建立的培养物在滚瓶中生长迅速，因此可以大量生产。这些细胞在液氮中储存3年后仍保持活力。
Finely minced explants from 54 TCC2 of the human urinary tract were cultured in vitro in an attempt to establish cell lines. Cells with epithelial morphology grew out from 48 tumor explants, and long-term cell cultures were established from 10. Six of the cell cultures have been maintained for over 18 months with 50 to 70 transfers and, therefore, are considered permanent cell lines. The epithelial cells in the established cultures are small, exhibit rapid doubling time, and show multilayering. The cells were examined both microscopically and by cultivation techniques, and they were found to be free from contaminating microorganisms, including Mycoplasma. The established cultures grow rapidly in roller bottles and, therefore, can be produced in large quantities. These cells also remain viable after being stored for 3 years in liquid nitrogen.