p66(Shc) Couples Mechanical Signals to RhoA through Focal Adhesion Kinase-Dependent Recruitment of p115-RhoGEF and GEF-H1
p66(Shc) Couples Mechanical Signals to RhoA through Focal Adhesion Kinase-Dependent Recruitment of p115-RhoGEF and GEF-H1
复制标题
p66(Shc) 通过 p115-RhoGEF 和 GEF-H1 的焦点粘附激酶依赖性招募将机械信号耦合到 RhoA
DOI：
10.1128/mcb.00194-16
复制
发表时间：
2016
影响因子：
5.3
通讯作者：
Terada Lance S.
中科院分区：
文献类型：
--
作者：
Wu Ru-Feng;Liao Chengxu;Fu Guosheng;Hayenga Heather N.;Yang Kejia;Ma Zhenyi;Liu Zhe;Terada Lance S.
Tissue cells respond to changes in tensional forces with proliferation or death through the control of RhoA. However, the response coupling mechanisms that link force with RhoA activation are poorly understood. We found that tension applied to fibronectin-coated microbeads caused recruitment of all three isoforms of the Shc adapter (p66Shc, p52Shc, and p46Shc) to adhesion complexes. The Shc PTB domain was necessary and sufficient for this recruitment, and screening studies revealed the direct interactions with the FERM domain of focal adhesion kinase (FAK) that were required for Shc translocation to adhesion complexes. The FAK/p66Shccomplex specifically bound and activated the Rho guanyl exchange factors (GEFs) p115-RhoGEF and GEF-H1, leading to tension-induced RhoA activation. In contrast, the FAK/p52Shccomplex bound SOS1 but not the Rho GEFs to mediate tension-induced Ras activation. Nuclear translocation and activation of the YAP/TAZ transcription factors on firm substrates required the FAK/p66Shc/Rho GEF complex, and both proliferation on firm substrates and anoikis in suspension required signaling through p66Shcand its associated Rho GEFs. These studies reveal the binary and exclusive assignment of p66Shcand p52Shcto tension-induced Rho or Ras signals, respectively, and suggest an integrated role for the two Shc isoforms in coordinating the cellular response to mechanical stimuli.
