Incorporation of single-wall carbon nanotubes into an organic polymer monolithic stationary phase for μ-HPLC and capillary electrochromatography
Incorporation of single-wall carbon nanotubes into an organic polymer monolithic stationary phase for μ-HPLC and capillary electrochromatography
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DOI：
10.1021/ac048299h
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发表时间：
2005-03-01
影响因子：
7.4
通讯作者：
Wilkins, JA
中科院分区：
文献类型：
--
作者：
Li, Y;Chen, Y;Wilkins, JA
将单壁碳纳米管（SWNT）嵌入到含有乙烯基苄基氯（VBC）和二甲基丙烯酸乙二酯（EDMA）的有机聚合物整体柱中，制备了一种新型的高效液相色谱（HPLC）和毛细管电色谱（CEC）整体柱固定相。采用微柱高效液相色谱法分离有机小分子混合物，考察了中性化合物在聚（VBC-EDMA-SWNT）整体柱上的保留行为。结果表明，SANT的掺入增强了反相HPLC中小分子的色谱保留，这可能是因为它们的强疏水特性。在熔融石英毛细管内形成固定相，该毛细管的内腔涂覆有共价结合的聚乙烯亚胺（PEI）。由PEI涂层产生的环形电渗流（EEP）允许通过CEC以反向模式分离肽。肽分离的聚（VBC-EDMA-单壁碳纳米管）和聚（VBC-EDMA）与环状双生成的比较表明，将单壁碳纳米管纳入整体固定相提高峰效率和影响色谱保留。采用高分辨透射电子显微镜、拉曼光谱、扫描电子显微镜和多点BET氮吸附/脱附等方法对预处理后的单壁碳纳米管和聚（VBC-EDMA-SWNT）整体结构进行了表征。
Single-wall carbon nanotubes (SWNT) were incorporated into an organic polymer monolith containing vinylbenzyl chloride (VBC)and ethylene dimethacrylate (EDMA) to form a novel monolithic stationary phase for high-performance liquid chromatography (HPLC) and capillary electrochromatography (CEC). The retention behavior of neutral compounds on this poly(VBC-EDMA-SWNT) monolith was examined by separating a mixture of small organic molecules using micro-HPLC. The result indicated that incorporation of SANT enhanced chromatographic retention of small neutral molecules in reversed-phase HPLC presumably because of their strongly hydrophobic characteristics. The stationary phase was formed inside a fused-silica capillary whose lumen was coated with covalently bound polyethyleneimine (PEI). The annular electroosmotic flow (EOF) generated by the PEI coating allowed peptide separation by CEC in the counterdirectional mode. Comparison of peptide separations on poly(VBC-EDMA-SWNT) and on poly(VBC-EDMA) with annular EOF generation revealed that the incorporation of SWNT into the monolithic stationary phase improved peak efficiency and influenced chromatographic retention. The structures of pretreated SWNT and poly(VBC-EDMA-SWNT) monolith were examined by high-resolution transmission electron microscopy, Raman spectroscopy, scanning electron microscopy, and multipoint BET nitrogen adsorption/desorption.