STRUCTURE AND ANTIGENIC ACTIVITY OF THE CAPSULAR POLYSACCHARIDE OF CRYPTOCOCCUS-NEOFORMANS SEROTYPE-A
STRUCTURE AND ANTIGENIC ACTIVITY OF THE CAPSULAR POLYSACCHARIDE OF CRYPTOCOCCUS-NEOFORMANS SEROTYPE-A
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DOI：
10.1016/0161-5890(80)90096-6
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发表时间：
1980-01-01
影响因子：
3.6
通讯作者：
BLUMER, SO
中科院分区：
文献类型：
--
作者：
CHERNIAK, R;REISS, E;BLUMER, SO
葡萄糖醛酸甘露聚糖（GXM）是C.新形式血清型A含有摩尔比为2：5：1：2的木糖：甘露糖：葡糖醛酸：O-乙酰基。木糖和葡萄糖醛酸作为单一的吡喃糖残基发生，并被高碘酸盐氧化;史密斯降解产物是一种不溶性甘露聚糖。甘露糖与5个甘露糖残基中的3个（1→2）连接-被一个葡萄糖醛酸基和两个单木糖基端基取代。一个重复的八糖提出的结构。GXM被分离成种特异性和菌型特异性组分，通过层析在一个caseype-D-抗体琼脂糖柱上，但这些单糖的摩尔比没有表现出明显的差异。血清学活性降低时，观察到GXM是去-O-乙酰化或羧基还原，但不是当羧基酯化。史密斯降解的甘露聚糖没有活性。自动水解引起木糖和一些甘露糖的逐渐释放，以及血清学活性的下降。木糖和葡萄糖醛酸被牵连在一个单一的抗原组，因为选择性失活的任何一个残基导致超过一半的活性损失。与C. laurentii和Tremella mesenterica，但不与II型或XIV型肺炎球菌多糖。
The glucuronoxylomannan (GXM) ofC. neoformansserotype A contained molar ratios of xylose: mannose: glucuronic acid:O-acetyl of 2:5:1:2. The xylose and glucuronic acid occurred as single pyranosyl residues and were oxidized by periodate; the Smith-degraded product was an insoluble mannan. Mannose was linked (1→3) with three out of five mannose residues (1→2)-substituted with one glucuronosyl and two single xylosyl end groups. A repeating octasaccharide was proposed as the structure. GXM was separated into species-specific and serotype-specific components by chromatography on a serotype-D-antibody agarose column, but these showed no obvious differences in molar ratios of the monosaccharides. Decreased serological activity was observed when GXM was de-O-acetylated or carboxy-reduced, but not when carboxy-esterified. The Smith-degraded mannan was inactive. Autohydrolysis caused a gradual release of xylose and some mannose, and a decline in serological activity. Xylose and glucuronic acid were implicated in a single antigenic group since selective inactivation of either residue led to loss of more than half of the activity. Extensive crossreactions were observed with the GXMs fromC. laurentiiandTremella mesenterica, but not with types II or XIV pneumococcal polysaccharides.