Effect of Head Size in Head-Tail-Type Polycations on their in vitro Performances as Nonviral Gene Vectors
Effect of Head Size in Head-Tail-Type Polycations on their in vitro Performances as Nonviral Gene Vectors
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DOI：
10.1002/mabi.200800314
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发表时间：
2009-06-11
影响因子：
4.6
通讯作者：
Kono, Kenji
中科院分区：
文献类型：
--
作者：
Harada, Atsushi;Kawamura, Masanori;Kono, Kenji
合成了三种由聚酰胺-胺（PAMAM）树枝头和聚赖氨酸（PLL）树枝尾组成的头尾型嵌段共聚物（PAMAM dendron-PLL），其中PAMAM树枝的代数不同（G2.5-PLL、G3.5-PLL和G4.5-PLL）。G2.5-PLL和G3.5-PLL可以形成小的复合物（小于150 nm）。G2.5-PLL和G3.5-PLL复合物被更有效地吸收到细胞中。具有较大树枝状头的PAMAM树枝状PLL可以表现出更有效的缓冲效果。PAMAM树枝状-PLL复合物的体外性能由细胞摄取和内体逃逸的平衡控制，缓冲作用。
Three kinds of head-tail-type block copolymers composed of polyamidoamine (PAMAM) dendron heads and poly(L-lysine) (PLL) tail blocks (PAMAM dendron-PLL), having PAMAM dendrons with different generations (G2.5-PLL, G3.5-PLL and G4.5-PLL) were synthesized. Some of the dendron heads were located at polyplex surface, and G2.5-PLL and G3.5-PLL could form small polyplexes (less than 150 nm in size). G2.5-PLL and G3.5-PLL polyplexes were taken up into the cells more effectively. PAMAM dendron-PLL that had a larger dendron head could show a more-effective buffering effect. The in vitro performance of the PAMAM dendron-PLL polyplexes was controlled by the balance of cellular uptake and endosomal escape by a buffering effect.