The chemical properties and hygroscopic activity of the exopolysaccharide lubcan from Paenibacillus sp. ZX1905.
The chemical properties and hygroscopic activity of the exopolysaccharide lubcan from Paenibacillus sp. ZX1905.
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DOI：
10.1016/j.ijbiomac.2020.08.129
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发表时间：
2020-08
影响因子：
8.2
通讯作者：
Xiaqing Sun;Lei Wang;Renjie Fu;Yunxia Yang;Rui Cheng;Jing Li;Shiming Wang;Jianfa Zhang
中科院分区：
文献类型：
--
作者：
Xiaqing Sun;Lei Wang;Renjie Fu;Yunxia Yang;Rui Cheng;Jing Li;Shiming Wang;Jianfa Zhang
本研究旨在探讨从土壤分离物类芽孢杆菌属中产生的胞外多糖（EPS）的生产和理化特性。 ZX1905 深层培养。在含有可溶性淀粉、蛋白胨和无机盐的培养基中，EPS 产量最高为 15.67 g/L。纯化后的EPS表现出优异的皮肤润滑性能，被命名为lubcan。化学分析表明，lubcan是一种酸性杂多糖，由葡萄糖醛酸、葡萄糖、甘露糖、半乳糖、鼠李糖按摩尔比2:3:1:2:2组成，平均分子量约为3.27×106Da。 NMR和甲基化分析表明lubcan主链由1,4-α-Man、1,4,6-α-Glc、1,3-α-Gal、1,3-β-Rha和1,3-β-Gal以及1,3-α-Glc、1,3-α-Rha、两个1,4-α-GlcA和1,3-α-Rha的分支组成。末端-α-Glc(4,6-pyr)。 Lubcan 溶液在 pH 值范围为 5.0 至 7.0、温度范围为 5 至 50 °C 以及添加一价盐 (0.2 M) 和二价盐 (0.05 M) 时表现出稳定性。在相对湿度43%和81%条件下，lubcan的吸湿率分别为16.98%和40.41%，与透明质酸（分别为17.28%和41.20%）接近。这些特性使 Lubcan 成为化妆品行业中更昂贵的透明质酸的良好替代品。
This study aims to explore the production and physicochemical properties of an exopolysaccharide (EPS) produced from soil isolate, Paenibacillus sp. ZX1905 in submerged culture. The highest EPS production of 15.67 g/L was achieved in a medium containing soluble starch, peptone and inorganic salts. The purified EPS exhibited excellent skin lubricating properties and was named lubcan. The chemical analysis reviewed that lubcan was an acidic heteropolysaccharide consisted of glucuronic acid, glucose, mannose, galactose, and rhamnose in a molar ratio of 2:3:1:2:2, and the average molecular mass was about 3.27 × 106Da. NMR and methylation analysis revealed that lubcan backbone was composed of 1,4-α-Man, 1,4,6-α-Glc, 1,3-α-Gal, 1,3-β-Rha, and 1,3-β-Gal, together with the branches of 1,3-α-Glc, 1,3-α-Rha, two 1,4-α-GlcA, and terminal-α-Glc(4,6-pyr). The lubcan solution exhibited stability at pH ranging from 5.0 to 7.0, temperature between 5 and 50 °C, and monovalent salts (0.2 M) and divalent salts (0.05 M) addition. The moisture absorption rates of lubcan were 16.98% and 40.41% under the conditions of 43% and 81% relative humidity, which were close to that of hyaluronic acid (17.28% and 41.20%, respectively). These properties make lubcan a good alternative to more expensive hyaluronic acid in the cosmetic industry.