STABILITY OF WATER-SOLUBLE CARBODIIMIDES IN AQUEOUS-SOLUTION
STABILITY OF WATER-SOLUBLE CARBODIIMIDES IN AQUEOUS-SOLUTION
复制标题
DOI：
10.1016/0003-2697(90)90675-y
复制
发表时间：
1990-02-01
影响因子：
2.9
通讯作者：
BORDERS, CL
中科院分区：
文献类型：
--
作者：
GILLES, MA;HUDSON, AQ;BORDERS, CL
用二甲基巴比尿酸试剂测定了两种水溶性碳二亚胺（1-乙基-3-（3-二甲氨基丙基）碳二亚胺（EDC）和结构相关的1-乙基-3-（4-偶氮-4,4-二甲基戊基）碳二亚胺（EAC））在水溶液中的损失动力学。在25度的50毫米2-(N-morpholino)乙磺酸中。在pH 7.0、6.0和5.0时，C、EDC的t1/2值分别为37、20和3.9 h，而EAC的t1/2值分别为12、2.9和0.32 h。在0.1 M时，碘化物、溴化物或氯化物对碳二亚胺的稳定性影响很小或没有影响。然而，0.1 M甘氨酸甲酯或0.1 M乙二胺会导致EAC和EDC的损失率显著增加，而0.1 M磷酸、0.1 M氢胺或0.01 M ATP的存在会将半衰期降低到0.1 M。在所有pH值下0.4 h。
A dimethylbarbituric acid reagent has been used to follow the kinetics of loss of two water-soluble carbodiimides, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and the structurally related 1-ethyl-3-(4-azonia-4,4-dimethylpentyl) carbodiimide (EAC), in aqueous solution as a function of pH and added chemical reagents. In 50 mM 2-(N-morpholino)ethanesulfonic acid at 25.degree. C, EDC has t1/2 values of 37, 20, and 3.9 h at pH 7.0, 6.0, and 5.0, respectively, while the corresponding values for EAC are 12,2.9, and 0.32 h. Iodide, bromide, or chloride, at 0.1 M, has very little or no effect on carbodiimide stability. However, 0.1 M glycine methyl ester or 0.1 M ethylenediamine causes a significant increase in the rate of loss of EAC and EDC, while the presence of 0.1 M phosphate, 0.1 m hydrosylamine, or 0.01 M ATP decreases the half-lives to .ltoreq. 0.4 h at all pH values.