AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging.
AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging.
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AQP5调节皮肤衰老过程中表皮干细胞的增殖和分化
DOI：
10.1590/1414-431x202010009
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Zhu N
中科院分区：
文献类型：
--
作者：
Zhou J;Dong Y;Liu J;Ren J;Wu J;Zhu N
表皮是皮肤的最外层，是与外部环境接触的第一道屏障。它在抵抗有害物质入侵和微生物感染方面起着重要作用。皮肤会随着年龄和外部环境因素而变化。本研究旨在探讨表皮干细胞在衰老过程中的作用。本研究招募了9名患有良性色素痣的志愿者进行临床皮肤科手术。测量与皮肤老化变化相关的表型，例如良性色素沉着皮肤附近的未暴露/健康部分的皮肤皱纹和弹性，并分离来自该区域的表皮干细胞用于转录组测序。结果表明，采用磁激活细胞分选法（MACS）可获得高纯度的表皮干细胞。转录组测序结果显示，随着年龄的增长，表皮干细胞中水通道蛋白（AQP）5的表达明显减少，进一步的功能实验显示，AQP 5可促进HaCaT的增殖和去分化，但不影响细胞凋亡。综上所述，AQP 5在皮肤衰老过程中调控表皮干细胞的增殖和分化，可能在维持增殖和分化的平衡中发挥重要作用。然而，需要进一步的研究来确定AQP 5在衰老过程中调节表皮皮肤细胞增殖和分化的机制。
The epidermis, the outermost layer of the skin, is the first barrier that comes into contact with the external environment. It plays an important role in resisting the invasion of harmful substances and microbial infections. The skin changes with age and external environmental factors. This study aimed to investigate epidermal stem cells during the process of aging. This study enrolled 9 volunteers with benign pigmented nevus for clinical dermatologic surgery. The phenotypes associated with skin aging changes such as skin wrinkles and elasticity of the unexposed/healthy parts near benign pigmented skin were measured, and epidermal stem cells from this region were isolated for transcriptome sequencing. The results showed that epidermal stem cells could be obtained by magnetic activated cell sorting (MACS) with high purity. Results of the transcriptome sequencing revealed that aquaporin (AQP)5 significantly decreased in the epidermal stem cells with age, and further functional experiments revealed that AQP5 could promote the proliferation and dedifferentiation of HaCaT, but did not influence cell apoptosis. In summary, AQP5 regulated the proliferation and differentiation of epidermal stem cells in skin aging, and it may play an important role in the balance of proliferation and differentiation. However, further studies are needed to determine the mechanism by which AQP5 regulates the proliferation and differentiation of epidermal skin cells in aging.