Loss of β-catenin expression associated with disease progression in malignant melanoma
Loss of β-catenin expression associated with disease progression in malignant melanoma
复制标题
DOI：
10.1046/j.1365-2133.2001.04336.x
复制
发表时间：
2001-08-01
影响因子：
10.3
通讯作者：
Ono, T
中科院分区：
文献类型：
--
作者：
Kageshita, T;Hamby, CV;Ono, T
目的检测β-连环蛋白在黑素细胞起源的皮损中的表达，探讨其与疾病进展的临床病理标志之间的关系，以及对疾病复发和预后的预测意义。方法采用免疫组织化学方法检测50例黑素细胞痣、91例原发黑色素瘤和50例转移性黑色素瘤中β-连环蛋白的表达，结果96%的黑素细胞痣、94%和65%的原发黑色素瘤和65%的转移性黑色素瘤和38%的转移性黑色素瘤表达。良性和恶性黑素细胞病变有不同的β-连环蛋白定位模式。然而，大多数表达β-连环蛋白的皮损呈胞浆染色。超过40%的良性病变也有核染色，只有10%的原发黑色素瘤和15%的转移性黑色素瘤有核染色。在原发性黑色素瘤中，β-连环蛋白的缺失或弱表达与疾病进展的几个标记物有关，包括肿瘤厚度和淋巴结转移的存在。结论在黑色素瘤细胞中，β-连环蛋白表达的缺失或下调在疾病的进展中起重要作用。
Background beta -catenin plays a crucial role in the function of cell adhesion molecules and also participates in growth regulatory signalling pathways that may be involved in malignant transformation.Objectives To examine beta -catenin expression in lesions of melanocytic origin for associations with clinicopathological markers of disease progression and for its significance as a predictor of disease recurrence and prognosis.Methods beta -catenin expression was examined by immunoperoxidase staining in 50 melanocytic naevi and 91 primary and 50 metastatic melanomas,Results beta -catenin was expressed in 96% of melanocytic naevi, in 94% and 65%, respectively, of radial and vertical growth phase primary melanomas, and in 38% of metastatic melanomas. Benign and malignant melanocytic lesions had distinct patterns of beta -catenin localization. Most lesions expressing beta -catenin exhibited cytoplasmic staining: however. over 40% of benign lesions also displayed nuclear staining, which was present only in 10% of primary and 15% of metastatic melanomas. Absent or weak expression of beta -catenin in primary melanomas was associated with several markers of disease progression, including tumour thickness and presence of lymph node metastases. A similar but not statistically significant trend was observed for the association of beta -catenin expression with disease recurrence and prognosis.Conclusions These results suggest that loss or downregulation of beta -catenin expression in melanoma cells plays a significant role in progression of the disease.