COMPARISON OF GLYCOPEPTIDES FROM TRANSFERRINS OF SEVERAL SPECIES
COMPARISON OF GLYCOPEPTIDES FROM TRANSFERRINS OF SEVERAL SPECIES
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DOI：
10.1042/bj1450263
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发表时间：
1975-01-01
影响因子：
4.1
通讯作者：
WILLIAMS, J
中科院分区：
文献类型：
--
作者：
GRAHAM, I;WILLIAMS, J
1.本文测定了人、猪、牛转铁蛋白和鸭卵转铁蛋白的碳水化合物组成。2.糖肽已从这些转铁蛋白和它们的碳水化合物组成和氨基酸序列测定。来自人转铁蛋白的糖肽之一携带蛋白质的C末端残基。3.每个转铁蛋白产生两个糖肽，似乎是相同的碳水化合物组成，但不同的氨基酸序列。这两种糖肽被区分为A型和B型，A型中Asn（CHO）（其中CHO代表碳水化合物部分）之后的残基是碱性氨基酸，B型中Asn（CHO）之后是中性脂肪族氨基酸。牛转铁蛋白具有两个糖肽，其中该位置被丝氨酸占据，这是例外。4.这表明，每个分子的人和牛的转铁蛋白和鸭卵转铁蛋白携带平均两个碳水化合物辅基。鸡和猪的转铁蛋白似乎每摩尔蛋白质只携带一个碳水化合物基团。5.还测定了鸡和鸭卵转铁蛋白以及牛、人和猪转铁蛋白的N-末端序列。
1. The carbohydrate compositions of human, pig and cattle transferrins and duck ovotransferrin have been determined. 2. Glycopeptides have been prepared from these transferrins and their carbohydrate compositions and amino acid sequences determined. One of the glycopeptides from human transferrin carries the C-terminal residue of the protein. 3. Each tranferrrin yielded two glycopeptides that appeared to be identical in carbohydrate composition but different in amino acid sequence. The two glycopeptides have been distinguished as type A, in which the residue following Asn(CHO)(where CHO represents a carbohydrate moiety) is a basic amino acid and type B in which Asn(CHO) is followed by a neutral aliphatic amino acid. Cattle transferrin is exceptional in having two glycopeptides in which this position is occupied by serine. 4. It is suggested that each molecule of human and cattle transferrin and duck ovotransferrin carries an average of two carbohydrate prosthetic groups. Hen and pig transferrins appear to carry only one carbohydrate group per mol of protein. 5. The N-terminal sequences of hen and duck ovotransferrins and of cattle, human and pig transferrins were also determined.