IMPLICATIONS OF ANTI-RO SJOGRENS SYNDROME-A ANTIGEN AUTOANTIBODY IN NORMAL SERA FOR AUTOIMMUNITY
IMPLICATIONS OF ANTI-RO SJOGRENS SYNDROME-A ANTIGEN AUTOANTIBODY IN NORMAL SERA FOR AUTOIMMUNITY
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DOI：
10.1172/jci112892
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发表时间：
1987-03-01
影响因子：
15.9
通讯作者：
HARLEY, JB
中科院分区：
文献类型：
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作者：
GAITHER, KK;FOX, OF;HARLEY, JB
我们用灵敏的方法分析了40例正常人血清中的7种Ro/Sjogren综合征自身抗体，其中7种结合了Ro/Sjogren综合征A抗原(SSA)，虽然这一结合比最高抗Ro/SSA水平低1000倍，但它可被人Ro/SSA抑制。亲和纯化的正常抗Ro/SSA Ig G可使特异性抗Ro/SSA结合增加17倍。纯化的抗体形成Ro/SSA沉淀素，与Ro/SSA沉淀素阳性患者的相对亲和力相同。这些数据表明，在健康的正常献血者中存在的抗Ro/SSA是真正的自身抗体。在40例正常人血清中，有3例检测到抗La/Sjogren‘S综合征B抗原自身抗体，而核核糖核蛋白(Sm)均不结合，在正常人中发现低水平的抗Ro/SSA和抗La/SsB，提示抗Ro/SSA和抗La/SsB可能是通过增强原有免疫反应而在疾病中发生的。
We have applied a sensitive assay to analyze lupus and Sjogren''s syndrome autoantibodies in 40 normal sera. Seven of these bound Ro/Sjogren''s syndrome A antigen (SSA). Although this binding was 1,000-fold lower than the highest anti-Ro/SSA level measured from patients, it was inhibited by human Ro/SSA. Positive normal serum-bound Ro/SSA in Western immunoblots and binding activity was demonstrated in the F(ab'')2 fragment of IgG. Affinity purification of normal anti-Ro/SSA IgG increased the specific anti-Ro/SSA binding by > 17-fold. This purified antibody formed a Ro/SSA precipitin and had a relative affinity for Ro/SSA identical to that of Ro/SSA precipitin-positive patients. These data demonstrate that the anti-Ro/SSA present in healthy normal donors is true autoantibody. Anti-La/Sjogren''s syndrome B antigen (SSB) autoantibodies were found in 3 of the 40 normal sera, while none bound nuclear ribonucleoprotein (Sm). Finding low levels of anti-Ro/SSA and anti-La/SSB among normals may indicate that anti-Ro/SSA and anti-La/SSB occur in disease by enhancement of a preexisting immune response.