DECREASED LEVELS OF C1Q IN CEREBROSPINAL-FLUID OF LIVING ALZHEIMER PATIENTS CORRELATE WITH DISEASE STATE
DECREASED LEVELS OF C1Q IN CEREBROSPINAL-FLUID OF LIVING ALZHEIMER PATIENTS CORRELATE WITH DISEASE STATE
复制标题
DOI：
10.1016/0197-4580(94)00055-7
复制
发表时间：
1994-09-01
影响因子：
4.2
通讯作者：
SHANKLE, WR
中科院分区：
文献类型：
--
作者：
SMYTH, MD;CRIBBS, DH;SHANKLE, WR
最近的报道表明，组成经典途径的补体蛋白与老年斑相关，这表明阿尔茨海默病组织中经典补体级联的激活导致旁观者细胞溶解，并可能参与AD的神经病理。对脑脊液的分析可能被证明是检测大脑免疫活动变化的有用手段。我们使用酶联免疫吸附试验来测量临床诊断为可能或可能患有AD的患者脑脊液中Clq的水平，Clq是经典补体级联的一个亚单位。AIzheimer组脑脊液中Clq水平显著低于对照组[AD：MU=268 ng/ml，SD=84；非AD：MU=340 ng/ml，SD=76；F(1，44)=5.84，P=0.02]。在诸如简易精神状态检查(R=0.45；p=0.0072)和福莱德的信息、记忆和注意力测试(R=0.42；p=0.0138)等全球精神状态测试中表现下降的表现与Clq水平的下降高度相关。更具体的认知功能测量，如单词回忆(R=0.42；p=0.012)、单词识别(R=0.52；p=0.0017)和延迟回忆(R=0.45；p=0.0062)记忆任务也与CLQ水平下降密切相关。
Recent reports that complement proteins comprising the classical pathway are associated with senile plaques suggest that activation of the classical complement cascade in Alzheimer's disease tissue results in bystander cell lysis and may contribute to AD neuropathology. Analysis of cerebrospinal fluid may prove to be a useful means of detecting changes in immunological activity in the brain. We use an enzyme-linked immunosorbent assay to measure levels of Clq, a subunit of the classical complement cascade, in the CSF of patients clinically diagnosed with possible or probable AD. Significantly lower levels of Clq were detected in the CSF of the AIzheimer group as compared to control CSF[AD: mu = 268 ng/ml, SD = 84; non-AD: mu = 340 ng/ml, SD = 76; F(1, 44) = 5.84, p = 0.02]. Diminished performance on global measures of mental status such as the Mini-Mental State Exam (R = 0.45; p = 0.0072) and Blessed's Information, Memory, and Concentration test (R = 0.42; p = 0.0138) showed high correlations with decreased Clq levels. More specific measures of cognitive function, such as word recall (R = 0.42; p = 0.012), word recognition (R = 0.52; p = 0.0017) and delayed recall(R = 0.45; p = 0.0062) memory tasks also correlated strongly with decreased Clq levels.