Effects of shikonin isolated from zicao on lupus nephritis in NZB/W F1 mice.
Effects of shikonin isolated from zicao on lupus nephritis in NZB/W F1 mice.
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DOI：
10.1248/bpb.32.1565
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发表时间：
2009-09
影响因子：
2
通讯作者：
Xin Chang Wang;Jian Feng;Feng Huang;Yong-Sheng Fan;Yan Yan Wang-Yan;Ling Cao;Cheng Wen
中科院分区：
文献类型：
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作者：
Xin Chang Wang;Jian Feng;Feng Huang;Yong-Sheng Fan;Yan Yan Wang-Yan;Ling Cao;Cheng Wen
本研究采用NZB/WF 1小鼠模型，观察紫草素对狼疮性肾炎（LN）的保护作用。将紫草素（24，40 mg/kg体重/d）或赋形剂口服给60只患有LN的28周龄雌性NZB/W F1小鼠。用紫草素治疗14周剂量依赖性地抑制蛋白尿，低剂量和高剂量紫草素组的平均蛋白尿分别为274.0 mg/dl和160.3 mg/dl，而赋形剂组为499.2 mg/dl。紫草素还能显著降低循环中粘附分子的含量，下调肾组织细胞间粘附分子-1（ICAM-1）和血管细胞粘附分子-1（VCAM-1）mRNA的表达。然而，与载体组不同，低或高剂量紫草素给药的小鼠中的抗双链（ds）DNA抗体均未表现出显著升高。肾脏组织学检查显示应用紫草素后肾小球病变减轻。这些结果提示紫草素对NZB/WF 1小鼠LN有治疗作用，抑制抗dsDNA抗体可能是其作用机制之一，其部分机制与抑制肾脏细胞粘附分子（CAM）mRNA表达有关。
The present study was performed to evaluate the potential protective effects of Shikonin extracted from Zicao on lupus nephritis (LN) using NZB/W F1 mice. Oral administration of Shikonin (24, 40 mg/kg body weight/d) or vehicle was applied to sixty female NZB/W F1 mice of 28-week-old with LN. Treatment with Shikonin for 14 weeks suppressed proteinuria dose-dependently with the mean proteinuria of 274.0 mg/dl and 160.3 mg/dl for low-dose and high-dose Shikonin groups, respectively, compared to 499.2 mg/dl for the vehicle. Also, Shikonin was observed to reduce circulating adhesion molecules significantly and down-regulate intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mRNA expression in kidney. However, anti-double stranded (ds)DNA antibody in mice with low or high Shikonin dose administration both exhibited no significant elevation, differing from vehicle group. Kidney histological examination showed that renal glomerular lesions were alleviated after Shikonin application. These results suggest that Shikonin has therapeutic effects on LN in NZB/W F1 mice, to which inhibition of anti-dsDNA may be potential contribution, and its part mechanism is related to suppression of mRNA expression of cell adhesion molecules (CAMs) in the kidney.