STRIATAL GRAFTS IN INFARCT STRIATOPALLIDUM INCREASE GABA RELEASE, REORGANIZE GABA, RECEPTOR AND IMPROVE WATER-MAZE LEARNING IN THE RAT
STRIATAL GRAFTS IN INFARCT STRIATOPALLIDUM INCREASE GABA RELEASE, REORGANIZE GABA, RECEPTOR AND IMPROVE WATER-MAZE LEARNING IN THE RAT
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DOI：
10.1016/0361-9230(94)90072-8
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发表时间：
1994-01-01
影响因子：
3.8
通讯作者：
NISHINO, H
中科院分区：
文献类型：
--
作者：
AIHARA, N;MIZUKAWA, K;NISHINO, H
我们将胎儿纹状体细胞移植到缺血大鼠模型中，并研究移植物的存活/生长、GABA 释放、GABA(A) 受体重组和功能恢复。大脑中动脉（MCA）一小时腔内闭塞诱发纹状体外侧部分和邻近皮层缺血性梗塞。在缺血大鼠中，莫里斯水迷宫学习的获得明显慢于对照大鼠。通过微透析检测，这些动物苍白球中的 GABA 水平约为对照组的一半。然而，在纹状体苍白球中移植胎儿纹状体细胞后，获取得到改善，因此在对照和移植动物的学习曲线的时间过程中没有观察到差异。 GABA 水平通过移植恢复到几乎正常水平。通过在灌注中使用 GABA 摄取阻滞剂（哌啶酸）处理，其进一步增加。在移植物中，使用[H-3]标记的SR95531放射自显影检测到的GABA(A)受体组织在移植后恢复了1年多。数据表明，梗塞纹状体中的胎儿纹状体细胞移植物可能部分重建纹状体苍白球GABA投射并重组GABA(A)受体。这可能是功能改进的基础。
We grafted fetal striatal cells in ischemic rat models, and investigated graft survival/growth, GABA release, GABA(A) receptor reorganization and functional recovery. One hour intraluminal occlusion of the middle cerebral artery (MCA) induced ischemic infarct in the lateral part of the striatum and adjacent cortex. In ischemic rats, the acquisition of Morris' water-maze learning was significantly slower than that of control rats. In these animals GABA level in the globus pallidus, detected by microdialysis, was about the half of that of controls. However, after the grafts of fetal striatal cells in the striatopallidum, the acquisition was improved, thus no difference was observed in the time course of learning curves in control and grafted animals. GABA level recovered to almost normal level by the graft. It further increased by the treatment of a GABA uptake blocker (nipecotic acids) in the perfusion. In the grafts, GABA(A) receptor organization detected by autoradiography using [H-3] labeled SR95531 was restored for more than 1 year after the graft. Data suggest that fetal striatal cell grafts in infarct striatum may partially reconstruct striatopallidal GABA projection and reorganize GABA(A) receptor. This might be a basis of improvement of function.