Alteration of elastin, collagen and their cross-links in abdominal aortic aneurysms
Alteration of elastin, collagen and their cross-links in abdominal aortic aneurysms
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DOI：
10.1053/ejvs.2002.1620
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发表时间：
2002-06-01
影响因子：
5.7
通讯作者：
Settembrini, PG
中科院分区：
文献类型：
--
作者：
Carmo, M;Colombo, L;Settembrini, PG
目的：尽管动脉扩张和动脉瘤形成的机制尚不清楚，但弹性蛋白和胶原的降解无疑起着至关重要的作用。我们通过检测动脉瘤和非动脉瘤腹主动脉壁中弹性蛋白和胶原的交联度来评价弹性蛋白和胶原的含量。材料和方法：用高效液相色谱法测定26例腹主动脉瘤手术标本和24例非动脉瘤腹主动脉尸检对照标本中弹性蛋白和胶原交联物的含量。用比色法测定4-羟基脯氨酸(4-HyPro)和5-羟基赖氨酸(5-hylys)的含量。结果：与对照组相比，动脉瘤组织中弹性蛋白交联物明显减少(桥粒和异桥粒减少90%，P<0.05)。
Objectives: although the mechanism of arterial dilation and aneurysm development has not been clarified, the degradation of elastin and collagen plays undoubtedly a critical role. We evaluated the elastin and collagen content through the detection of their cross-links in aneurysmal and non-aneurysmal abdominal aortic walls.Materials and methods: in 26 human abdominal aortic aneurysm specimens obtained during surgery and in 24 autopsy control samples of non-aneurysmal abdominal aorta the tissue content of elastin and collagen cross-links were measured by HPLC. Collagen was also detected by evaluating two characteristic amino acids, 4-hydroxyproline (4-hypro) with a colorimetric method and 5-hydroxylysine (5-hylys) by gas chromatography.Results: significantly fewer elastin cross-links were found in aneurysm samples compared to controls (desmosines and isodesmosines: 90% reduction; p