Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations
Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations
复制标题
DOI：
10.1016/j.devcel.2014.11.029
复制
发表时间：
2014-12-22
影响因子：
11.8
通讯作者：
Cohen, Stephen M.
中科院分区：
文献类型：
--
作者：
Chen, Ya-Wen;Song, Shilin;Cohen, Stephen M.
MicroRNAs在动物基因组中含量丰富，但对其在体内的功能知之甚少。在这里，我们报道了通过定向同源重组产生80个新的果蝇miRNA突变体。这些突变体移除了104个miRNA。加上之前报道的15个突变体，这个集合包括95个突变体，缺失130个miRNAs。通过miRNA序列读取，这些基因总共产生了所有果蝇miRNAs的99%以上。我们对发育和成体miRNA的表型进行了调查。超过80%的突变体在P<；0.01显著阈值下至少表现出一种表型。我们观察到miRNA丰度与与生存和寿命相关的表型显著相关，但与大多数其他表型无关。MiRNA簇突变体并不比单个miRNA突变体更有可能产生显著的表型。这一突变体的收集将为未来分析果蝇miRNAs的生物学作用提供资源。
MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deleting 130 miRNAs. Collectively, these genes produce over 99% of all Drosophila miRNAs, measured by miRNA sequence reads. We present a survey of developmental and adult miRNA phenotypes. Over 80% of the mutants showed at least one phenotype using a p < 0.01 significance threshold. We observed a significant correlation between miRNA abundance and phenotypes related to survival and lifespan, but not to most other phenotypes. miRNA cluster mutants were no more likely than single miRNA mutants to produce significant phenotypes. This mutant collection will provide a resource for future analysis of the biological roles of Drosophila miRNAs.