ASYMMETRIC SPIKE-TIMING DEPENDENT PLASTICITY OF STRIATAL NITRIC OXIDE-SYNTHASE INTERNEURONS
ASYMMETRIC SPIKE-TIMING DEPENDENT PLASTICITY OF STRIATAL NITRIC OXIDE-SYNTHASE INTERNEURONS
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DOI：
10.1016/j.neuroscience.2009.03.015
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发表时间：
2009-06-02
期刊：
影响因子：
3.3
通讯作者：
Venance, L.
中科院分区：
文献类型：
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作者：
Fino, E.;Paille, V.;Venance, L.
皮质纹状体投射构成基底神经节的主要输入，基底神经节是皮层下核的集合，参与对环境刺激的认知-运动序列的学习。除了纹状体输出神经元（中型棘神经元，MSNs）负责检测皮层活动外，三大类中间神经元（GABAergic， cholininergic和一氧化氮（NO）合成酶中间神经元）密切调节皮质纹状体信息传递。尽管一氧化氮在神经元信号传导和突触可塑性中起着至关重要的作用，但在纹状体神经元一氧化氮合酶（nNOS）中间神经元水平上皮质纹状体突触传递和可塑性的研究却很少。利用保留体感觉皮层和纹状体细胞之间连接的皮质纹状体脑切片，我们探索了大脑皮层和纹状体nNOS中间神经元之间的突触传递，以及它们基于皮质和纹状体准重合活动发展活动依赖的长期可塑性的能力（spike-timing dependent plasticity， STDP）。我们观察到皮层锥体细胞单突触激活纹状体nNOS中间神经元非常有效。此外，根据STDP协议，nNOS中间神经元能够发展出强大的双向长期可塑性。事实上，nNOS中间神经元皮层诱发反应的强度随突触前和突触后激活之间的时间间隔而变化（δ t=t（后）-t（前））。对于t0， EPSCs在0时被抑制
Corticostriatal projections constitute the major inputs to basal ganglia, an ensemble of sub-cortical nuclei involved in the learning of cognitive-motor sequences in response to environmental stimuli. Besides striatal output neurons (medium-sized spiny neurons, MSNs) in charge of the detection of cortical activity, three main classes of interneurons (GABAergic, cholinergic and nitric oxide (NO)-synthase interneurons) tightly regulate the corticostriatal information transfer. Despite the crucial role of NO on neuronal signaling and synaptic plasticity, little is known about corticostriatal synaptic transmission and plasticity at the level of striatal neuronal nitric oxide synthase (nNOS) interneurons. Using a corticostriatal rat brain slice preserving the connections between the somatosensory cortex and the striatal cells, we have explored the synaptic transmission between the cerebral cortex and striatal nNOS interneurons and their capability to develop activity-dependent long-term plasticity based on the quasi-coincident cortical and striatal activities (spike-timing dependent plasticity, STDP). We have observed that cortical pyramidal cells activate monosynaptically and very efficiently the striatal nNOS interneurons. In addition, nNOS interneurons are able to develop strong bidirectional long-term plasticity, following STDP protocols. Indeed, the strength of cortically-evoked response at nNOS interneurons varied as a function of time interval between pre- and postsynaptic activations (Delta t=t(post)-t(pre)). For Delta t0, EPSCs depressed for 0