Effects of Testicular and Ovarian Inhibin‐Like Activity, Using In Vitro and In Vivo Systems
Effects of Testicular and Ovarian Inhibin‐Like Activity, Using In Vitro and In Vivo Systems
复制标题
使用体外和体内系统对睾丸和卵巢抑制素样活性的影响
DOI：
10.1111/j.1365-2605.1978.tb00011.x
复制
发表时间：
1978
影响因子：
--
通讯作者：
H. J. Molen
中科院分区：
文献类型：
--
作者：
F. Jong;R. Welschen;W. P. Hermans;S. Smith;H. J. Molen
textabstract在体外生物测定系统中，使用培养的垂体细胞，测定了活性炭处理的牛卵泡液（FF）和培养的支持细胞（SCCM）培养基中的抑制素样活性。在存在或不存在促黄体激素释放激素（LH-RH）的情况下，添加两种液体均导致培养基中促卵泡激素（FSH）浓度平行剂量依赖性降低。未成熟和成年雄性和雌性大鼠单次注射FF后4或8 h，外周FSH水平降低，但LH水平未降低。成年雌性大鼠的这种下降幅度大于青春期前雌性动物，且下降速度更快。单侧卵巢切除术（ULO）后立即将FF注射到成年雌性大鼠中，可防止对照动物中FSH水平的特异性升高。类固醇治疗后不能获得这种抑制。成年雌性或未成熟雄性大鼠每日给药超过5天，不会导致循环FSH浓度的长期抑制; LH水平显著升高。雌性动物显示周期性阴道涂片变化和正常排卵;雄性大鼠睾丸重量和生精细胞数量减少。得出的结论是，睾丸和卵巢抑制素样活性具有相似的性质。向雄性和雌性大鼠注射FF对FSH和LH产生相似的影响。FF在ULO动物中的作用表明，Escherichin可能在卵巢中发育卵泡数量的短期调节中发挥作用。将FF注射到雄性大鼠中可能通过FSH的初始抑制引起精子发生的短暂损伤。版权
textabstractInhibin‐like activities in charcoal‐treated bovine follicular fluid (FF) and medium from cultured Sertoli cells (SCCM) were assayed in an in vitro bioassay system, using cultured pituitary cells. Addition of both fluids resulted in parallel dose‐dependent decreases of the concentration of follicle‐stimulating hormone (FSH) in the medium, both in the presence or absence of luteinizing hormone‐releasing hormone (LH‐RH). A single injection of FF into immature and adult male and female rats resulted in decreased peripheral levels of FSH, but not of LH, after 4 or 8 h. This decrease was larger and occurred faster in adult female rats than in prepubertal female animals. Injection of FF into adult female rats, immediately after unilateral ovariectomy (ULO) prevented the specific increase of FSH levels, occurring in control animals. This suppression could not be obtained after treatment with steroids. Daily treatment of adult female or immature male rats for periods longer than 5 days did not result in prolonged suppression of circulating FSH concentrations; LH levels were significantly increased. The female animals showed cyclic vaginal smear changes and normal ovulation; in the male rats testis weight and numbers of spermatogenic cells were reduced. It is concluded that testicular and ovarian inhibin‐like activities have similar properties. Injections of FF into male and female rats cause similar effects on FSH and LH. The effect of FF in ULO‐animals suggests that inhibin could play a role in the short‐term regulation of the number of developing follicles in the ovary. Injection of FF into male rats causes a probably transient impairment of spermatogenesis through an initial suppression of FSH. Copyright