MICROIONTOPHORESIS OF COCAINE, DESIPRAMINE, SULPIRIDE, METHYSERGIDE, AND NALOXONE IN HABENULA AND PARAFASCICULUS
MICROIONTOPHORESIS OF COCAINE, DESIPRAMINE, SULPIRIDE, METHYSERGIDE, AND NALOXONE IN HABENULA AND PARAFASCICULUS
复制标题
DOI：
10.1016/0014-4886(90)90129-g
复制
发表时间：
1990-06-01
影响因子：
5.3
通讯作者：
DAFNY, N
中科院分区：
文献类型：
--
作者：
DOUGHERTY, PM;QIAO, JT;DAFNY, N
本实验观察了微电泳可卡因、地昔帕明（DES）、舒必利（SUL）、甲基麦角酰胺（METH）和纳洛酮（NAL）对大鼠缰核（Hab）和丘脑束旁核（PF）内生理性单个神经元反应的影响。根据伤害性刺激后获得的反应，在两个细胞核中鉴定出三种细胞类型，它们被分类为“伤害性感受性开启”、“伤害性感受性关闭”和“非伤害性感受性”细胞。可卡因的管理一般导致伤害感受性和nonnociceptive神经元在HAB和PF的放电率下降。相反，可卡因一般诱导的伤害感受性关闭细胞的基线放电的兴奋。与伤害性刺激同时应用的coconut阻止了伤害性感受开细胞和伤害性感受关细胞的诱发反应。DES，单独应用时，发现在两个网站的所有三种类型的细胞的神经元放电中诱导兴奋。可卡因与DES的联合应用导致在放电频率没有观察到的变化的伤害性感受和nonnociceptive细胞，而诱导的伤害性感受关闭细胞的附加兴奋作用。相比之下，SUL单独给药时对基线放电没有明显影响，但始终拮抗可卡因对所有三种细胞类型的影响。最后，METH和NAL对基线放电或可卡因引起的神经元放电频率的改变没有影响。
The effects of microiontophoretically applied cocaine, desipramine (DES), sulpiride (SUL), methylsergide (METH), and naloxone (NAL) on the responses of physiologically identified single neurons in the habenula (Hab) and parafasciculus thalami nucleus (PF) were examined in rats. Three cell types were identified in both nuclei on the basis of the responses obtained following noxious stimulation that were classified as "nociceptive-on," "nociceptive-off," and "nonnociceptive" cells. Administration of cocaine generally resulted in a decrease in the firing rate of nociceptive-on and nonnociceptive neurons in both Hab and PF. In contrast, cocaine generally induced an excitation in the baseline firing of the nocicpetive-off cells. Cocaine application concomitant with noxious stimulation prevented the evoked responses of the nociceptive-on and the nociceptive-off cells. DES, when applied alone, was found to induce excitation in neuronal discharge of all three cell types in both sites. Combined application of cocaine with DES resulted in no observable change in discharge frequency for the nociceptive-on and nonnociceptive cells, while inducing an additive excitatory effect on the nociceptive-off cells. SUL, in contrast, induced no observable effect on baseline firing when given alone, yet consistently antagonized cocaine-induced effects on all three cell types. Finally, METH and NAL induced no effects on baseline firing or cocaine-induced modifications in neuronal discharge frequency.