Metabolomic comparison between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris
Metabolomic comparison between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris
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DOI：
10.1002/bmc.4279
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发表时间：
2018-09-01
影响因子：
1.8
通讯作者：
Zhang, Wancun
中科院分区：
文献类型：
--
作者：
Chen, Lin;Liu, Yuetao;Zhang, Wancun
采用液相色谱-质谱联用技术对野生冬虫夏草与人工养殖蛹虫草的代谢组差异进行了系统研究。主成分分析和潜在结构判别分析的正交投影结果表明，在固体水稻培养基（R-CM）和柞蚕蛹培养基（T-CM）上生长的蛹螟与野生柽柳有明显的分离和单独分离，表明野生柽柳、R-CM和T-CM之间存在代谢组差异。R-CM与T-CM的代谢组差异表明，军伞草可以通过不同的代谢调节来适应培养基。进一步进行分层聚类分析，根据代谢相似性对差异代谢物和样品进行聚类。其氨基酸（焦谷氨酸、谷氨酸、组氨酸、苯丙氨酸和精氨酸）、不饱和脂肪酸（亚麻酸和亚油酸）、多肽、甘露醇、腺苷和琥珀酸腺苷含量较高，是中药补益和滋补的理想选择。人工培养的军国蓟与中华玉蓟组成相似，且易于栽培，是中华玉蓟的可能替代品。
A systematic study on the metabolome differences between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris was conducted using liquid chromatography-mass spectrometry. Principal component analysis and orthogonal projection on latent structure-discriminant analysis results showed that C. militaris grown on solid rice medium (R-CM) and C. militaris grown on tussah pupa (T-CM) evidently separated and individually separated from wild O. sinensis, indicating metabolome difference among wild O. sinensis, R-CM and T-CM. The metabolome differences between R-CM and T-CM indicated that C. militaris could accommodate to culture medium by differential metabolic regulation. Hierarchical clustering analysis was further performed to cluster the differential metabolites and samples based on their metabolic similarity. The higher content of amino acids (pyroglutamic acid, glutamic acid, histidine, phenylalanine and arginine), unsaturated fatty acid (linolenic acid and linoleic acid), peptides, mannitol, adenosine and succinoadenosine in O. sinensis make it as an excellent choice as a traditional Chinese medicine for invigoration or nutritional supplementation. Similar compositions with O. sinensis and easy cultivation make artificially cultured C. militaris a possible alternative to O. sinensis.