Studies on the mechanism of decreased NMR-measured free magnesium in stored erythrocytes.
Studies on the mechanism of decreased NMR-measured free magnesium in stored erythrocytes.
复制标题
研究核磁共振测量的储存红细胞中游离镁减少的机制。
DOI：
10.1016/0167-4889(87)90079-6
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Gupta,RK
中科院分区：
文献类型：
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作者：
Bock,JL;Wenz,B;Gupta,RK
在4°C下储存于各种保存介质中的红细胞上记录了31 P-NMR光谱。贮存总是与无机磷酸盐（Pi）共振的高场位移和ATPβ共振的显著高场位移相关，表明细胞内pH（pHi）降低和细胞内游离镁（[Mg 2 +]i）降低。在不含柠檬酸盐的保存培养基中，甚至在补充有Mg ~（2+）的保存培养基中，[Mg ~（2+）] i都有所降低。这不能归因于储存过程中发生的pHi、Na+、K+、乳酸盐、Pior 2，3-二磷酸甘油酸盐的变化。当将储存的细胞在新鲜血浆中于37°C孵育1小时时，[Mg 2 +] i的降低在很大程度上逆转。发现储存的细胞含有大量的无机焦磷酸盐，每升细胞水高达约200 μmol。作为Mg 2+的紧密结合剂，焦磷酸盐可以解释所观察到的[Mg 2 +]i的一些降低。另外的机制可能涉及在冷藏过程中一些其它Mg 2+络合物的沉淀或Mg 2+与膜组分结合的增强。
31P-NMR spectra have been recorded on erythrocytes stored at 4°C in various preservation media. Storage was always associated with an upfield shift of the inorganic phosphate (Pi) resonance and a pronounced upfield shift of the ATPβresonance, indicating decreased intracellular pH (pHi) and decreased intracellular free magnesium ([Mg2+]i). The decreased [Mg2+]ioccurred in preservation media not containing citrate and even in media supplemented with Mg2+. It could not be attributed to the changes in pHi, Na+, K+, lactate, Pior 2,3-diphosphoglycerate, that occur with storage. The decrease in [Mg2+]iwas largely reversed when stored cells were incubated for 1 h at 37°C in fresh plasma. Stored cells were found to contain significant amounts of inorganic pyrophosphate, up to about 200 μmol per liter cell water. Being a tight binder of Mg2+, pyrophosphate could account for some of the observed decrease in [Mg2+]i. Additional mechanisms may involve precipitation of some other Mg2+complex during cold storage or enhancement of Mg2+binding to membrane components.