Differential effects of luminol, nickel, and arsenite on the rejoining of ultraviolet light and alkylation‐induced DNA breaks
Differential effects of luminol, nickel, and arsenite on the rejoining of ultraviolet light and alkylation‐induced DNA breaks
复制标题
鲁米诺、镍和亚砷酸盐对紫外线重新连接和烷基化诱导的 DNA 断裂的不同影响
DOI：
10.1002/em.2850230207
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发表时间：
1994
影响因子：
2.8
通讯作者：
K. Jan
中科院分区：
文献类型：
--
作者：
S. Lee;C. Yu;D. R. Wu;K. Jan
用紫外线(UV)或甲基磺酸甲酯(MMS)处理中国仓鼠卵巢细胞后，碱性洗脱可检测到大量DNA断裂。如果让处理过的细胞在无药物的介质中恢复，这些链断裂就会逐渐消失。在恢复孵育过程中，镍或亚砷酸盐的存在延缓了紫外线诱导的链断裂的消失，而亚砷酸盐或新的聚(ADP-核糖)合成酶抑制剂鲁米诺的存在则延缓了MMS诱导的链断裂的消失。鲁米诺对紫外线诱导的DNA链损伤修复无明显作用，镍对MMS诱导的DNA链损伤修复无明显影响。UV或MMS处理的细胞在胞嘧啶阿拉伯糖苷(AraC)+羟基脲(HU)中孵育后，碱性蔗糖沉淀法检测到大量的小分子DNA。如果细胞进一步在无药物的培养液中孵育，这些DNA的分子量就会增加。在紫外线加AraC和HU处理的细胞中，这种断裂的重新连接可以被镍和亚砷酸盐抑制，但不能被鲁米诺抑制。在MMS+AraC和HU处理的细胞中，断裂的再连接可被鲁米诺和亚砷酸盐抑制，但不能被镍抑制。这些结果表明，在UV和MMS诱导的DNA链断裂修复过程中，不同的酶可能用于DNA的再合成和/或连接，镍、鲁米诺和亚砷酸盐对这些酶可能有不同的抑制作用。©1994 Wiley-Liss，Inc.
When Chinese hamster ovary cells were treated with ultraviolet (UV) light or methyl methanesulfonate (MMS), a large number of DNA strand breaks could be detected by alkaline elution. These strand breaks gradually disappeared if the treated cells were allowed to recover in a drug‐free medium. The presence of nickel or arsenite during the recovery incubation retarded the disappearance of UV‐induced strand breaks, whereas the disappearance of MMS‐induced strand breaks was retarded by the presence of arsenite or of luminol, a new inhibitor for poly(ADP‐ribose) synthetase. Luminol, however, had no apparent effect on the repair of UV‐induced DNA strand breaks, and nickel had no effect on the repair of MMS‐induced DNA strand breaks. When UV‐ or MMS‐treated cells were incubated in cytosine arabinofuranoside (AraC) plus hydroxyurea (HU), a large amount of low molecular weight DNA was detected by alkaline sucrose sedimentation. The molecular weight of these DNAs increased if the cells were further incubated in a drug‐free medium. This rejoining of breaks in cells pretreated with UV plus AraC and HU was inhibited by nickel and by arsenite, but not by luminol. The rejoining of breaks in cells pretreated with MMS plus AraC and HU was inhibited by luminol and by arsenite, but not by nickel. These results suggest that different enzymes may be used in DNA resynthesis and/or ligation during the repairing of UV‐ and MMS‐induced DNA strand breaks, and that nickel, luminol, and arsenite may have differential inhibitory effects on these enzymes. © 1994 Wiley‐Liss, Inc.
根据细胞类型，聚（ADP-核糖）修复烷化剂和紫外线造成的 DNA 损伤的调节存在差异。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cleaver,JE;Bodell,WJ;Morgan,WF;Zelle,B
通讯作者：
Zelle,B
对用于研究哺乳动物 DNA 修复的透化细胞系统的批判性评论。
DOI：
10.1016/0921-8777(90)90008-s
发表时间：
1990
期刊：
Mutation research
影响因子：
--
作者：
Keeney,S;Linn,S
通讯作者：
Linn,S
体外转录：全细胞提取物。
影响因子：
--
作者：
Manley,JL;Fire,A;Samuels,M;Sharp,PA
通讯作者：
Sharp,PA