Identification of ESM1 overexpressed in head and neck squamous cell carcinoma
Identification of ESM1 overexpressed in head and neck squamous cell carcinoma
复制标题
头颈鳞状细胞癌中过表达 ESM1 的鉴定
DOI：
10.1186/s12935-019-0833-y
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发表时间：
2019-05-02
影响因子：
5.8
通讯作者：
Huang, Zhigang
中科院分区：
文献类型：
--
作者：
Xu, Hongbo;Chen, Xiaohong;Huang, Zhigang
背景Endocan又称内皮细胞特异性分子-1（endothelial cell specific molecule-1，ESM 1），是一种分子量为50 kDa的可溶性蛋白多糖，在多种肿瘤中表达频繁。它是否是失调，在头颈部鳞状细胞癌（HNSCC）还没有investigated.MethodsWe分析了ESM 1的表达，使用生物信息学分析的基础上，从癌症基因组图谱（TCGA）的数据，然后验证，ESM 1是显着过表达的人HNSCC在蛋白质水平上使用免疫组化。我们还分析了与ESM 1在HNSCC.ResultsThe最相关的基因是血管生成素-2（ANGPT 2），一种调节生理和病理血管生成的分子。在与ESM 1共表达的基因的启动子区域中，包括SMAD 3、SMAD 4、SOX 3、SOX 4、HIF 2A和AP-1组分的几种转录因子结合基序显著富集。进一步分析的基础上ChIP-seq数据从ENCODE（DNA元素的百科全书）项目显示，AP-1是一个重要的调节ESM 1 expression.ConclusionsOur结果揭示了ESM 1的失调和潜在的监管机制的共表达网络在HNSCC。
BackgroundEndocan, also known as endothelial cell specific molecule-1 (ESM1), is a 50kDa soluble proteoglycan which is frequently overexpressed in many cancer types. Whether it is dysregulated in head and neck squamous cell carcinoma (HNSCC) has not been investigated.MethodsWe analyzed the expression of ESM1 using bioinformatics analysis based on data from The Cancer Genome Atlas (TCGA), and then validated that ESM1 was significantly overexpressed in human HNSCC at the protein level using immunohistochemistry. We also analyzed the genes co-expressed with ESM1 in HNSCC.ResultsThe most correlated gene was angiopoietin-2 (ANGPT2), a molecule which regulates physiological and pathological angiogenesis. Several transcription factor binding motifs including SMAD3, SMAD4, SOX3, SOX4, HIF2A and AP-1 components were significantly enriched in the promoter regions of the genes co-expressed with ESM1. Further analysis based on ChIP-seq data from the ENCODE (Encyclopedia of DNA Elements) project revealed that AP-1 is an important regulator of ESM1 expression.ConclusionsOur results revealed a dysregulation of ESM1 and a potential regulatory mechanism for the co-expression network in HNSCC.