Influence of Intra-articular Neutrophils on the Effects of Photodynamic Therapy for Murine MRSA Arthritis
Influence of Intra-articular Neutrophils on the Effects of Photodynamic Therapy for Murine MRSA Arthritis
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DOI：
10.1111/j.1751-1097.2009.00658.x
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发表时间：
2010-03-01
影响因子：
3.3
通讯作者：
Morimoto, Yuji
中科院分区：
文献类型：
--
作者：
Tanaka, Masamitsu;Kinoshita, Manabu;Morimoto, Yuji
虽然已经有一些关于光动力疗法（PDT）对多药耐药细菌的细胞毒性作用的报道，但是很少有报道描述PDT对局部感染部位的有利结果。本研究旨在验证PDT对局部感染部位的低疗效是由于PDT对白细胞的细胞毒性作用的假设。使用Photofrin（R）的PDT对培养的耐甲氧西林金黄色葡萄球菌（MRSA）具有显著的细胞毒性。然而，这种治疗方式对鼠MRSA关节炎模型无效。大约30%的关节内白细胞，主要是中性粒细胞，PDT后立即死亡，PDT后24小时关节内白细胞数量进一步减少，滑膜组织萎缩。分离的外周嗜中性粒细胞显示出对Photofrin（R）的显著亲和力，并显示出显著的形态学损伤，当它们经受使用Photofrin（R）的PDT时，导致细胞死亡。这些结果表明关节内中性粒细胞对PDT治疗MRSA关节炎的效果有影响。
Although there have been some reports about the cytotoxic effects of photodynamic therapy (PDT) on multidrug-resistant bacteria, there have been few reports in which favorable results of PDT on a local infection site are described. This study aimed to verify the hypothesis that the low efficacy of PDT on a local infection site is due to the cytotoxic effect of PDT on leukocytes. PDT using Photofrin (R) exerted significant cytotoxicity for cultured methicillin-resistant Staphylococcus aureus (MRSA). Nevertheless, this therapeutic modality was not effective for a murine MRSA arthritis model. Approximately 30% of intra-articular leukocytes, mainly neutrophils, died immediately after PDT, and a further decrease in the number of intra-articular leukocytes and atrophy of the synovial tissue were seen 24 h after PDT. Isolated peripheral neutrophils showed significant affinity for Photofrin (R) and showed significant morphological damage, resulting in cell death, when they were subject to PDT using Photofrin (R). These results indicate that intra-articular neutrophils have an influence on the effects of PDT for MRSA arthritis.