The effect of PKC activity on the TTX-R sodium currents from rat nodose ganglion neurons
The effect of PKC activity on the TTX-R sodium currents from rat nodose ganglion neurons
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DOI：
10.1016/j.lfs.2005.04.053
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发表时间：
2005-11-19
期刊：
影响因子：
6.1
通讯作者：
Mastumoto, S
中科院分区：
文献类型：
--
作者：
Ikeda, M;Yoshida, S;Mastumoto, S
为了确定蛋白激酶C（PKC）活性如何影响新生大鼠结状神经节（NG）神经元中河豚毒素抗性钠电流（TTX-R I-Na）的性质，我们评估了佛波醇、-12-肉豆蔻酸酯、13 -乙酸酯（PMA）（PKC激活剂之一）和星形孢菌素（PKC抑制剂之一）对电流的影响。PMA（30和100 nM）诱导的归一化电流-电压曲线的峰值电流幅度的增加，在归一化的电导-电压曲线的半激活（VI 2）和稳态失活曲线的V-1/2的电位的一个单位位移的电位。星形孢菌素（0. 1 μ M和1 μ M）对峰值电流幅值和V-1/2激活电位的影响与PMA应用后所观察到的相反。星形孢菌素（1 μ M）拮抗PMA（100 nM）诱导的TTX-R IN修饰。这些结果表明，从新生NG神经元获得的基础TTX-R IN是由PKC活性水平控制的。(c)2005年爱思唯尔公司All rights reserved.
To determine how protein kinase C (PKC) activity influences properties of the tetrodotoxin-resistant sodium current (TTX-R I-Na) in neonatal rat nodose ganglion (NG) neurons, we assessed the effects of phorbol,- 12-myristate, 13 -acetate (PMA), one of the PKC activators, and staurosporine, one of the PKC inhibitors, on the current. PMA (30 and 100 nM) induced an increase in the peak current amplitude of normalized current-voltage curves, a leftward shift in the potential for half activation (VI 2) of normalized conductance- voltage curves and a leftward shift of V-1/2 potential for steady-state inactivation curves. The effects of staurosporine (0. 1 and 1 mu M) on the peak current amplitude and the V-1/2 potential for activation were opposite compared with those seen after PMA application. Staurosporine (I mu M) antagonized PMA (100 nM)-induced modification of TTX-R IN,. These results suggest that the basal TTX-R IN obtained from neonatal NG neurons is controlled by the level of PKC activity. (c) 2005 Elsevier Inc. All rights reserved.