Use of a glass bead-containing liquid medium for efficient production of a soil-free culture with polychlorinated biphenyl-dechlorination activity
Use of a glass bead-containing liquid medium for efficient production of a soil-free culture with polychlorinated biphenyl-dechlorination activity
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DOI：
10.1007/s11274-013-1310-8
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发表时间：
2013-03
影响因子：
4.1
通讯作者：
D. Suzuki;D. Baba;V. Satheeja Santhi;R. D. Jebakumar Solomon;A. Katayama
中科院分区：
文献类型：
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作者：
D. Suzuki;D. Baba;V. Satheeja Santhi;R. D. Jebakumar Solomon;A. Katayama
我们建立了一个无土培养能够脱氯多氯联苯（PCBs）在Kanechlor-300和Kanechlor-400通过建立一个PCBs脱氯土壤培养液中含有0.5毫米的玻璃珠。多氯联苯-脱氯活性的液体培养物与玻璃珠似乎取决于大小的玻璃珠，和无土培养物与0.05-，1.0-或2.0毫米的玻璃珠没有脱氯多氯联苯。没有玻璃珠的无土培养也未能使多氯联苯脱氯。含有0.5 mm玻璃珠的无土培养物对PCBs的脱氯率为42.6 ± 12.0 mol%。这种无土培养比土壤培养对从二氯到四氯多氯联苯的多氯联苯的脱氯效果更好。16 S rRNA基因序列的克隆分析表明，在无土培养的微生物的主要群体之一，包括耐热和孢子形成的细菌从厚壁菌门。热处理（100 °C，10分钟）没有破坏的多氯联苯脱氯活性的无土培养与玻璃珠。这些结果表明，在无土培养中，厚壁菌门的未知物种参与了PCB的脱氯。在这项研究中，我们成功地使用含有玻璃珠的液体介质作为无机土壤替代品，并表明，这样的介质增强多氯联苯脱氯活性。本研究为开发利用脱氯细菌修复缺氧污染土壤和沉积物中多氯联苯的生物修复技术提供了有价值的信息。
We established a soil-free culture capable of dechlorinating polychlorinated biphenyls (PCBs) in Kanechlor-300 and Kanechlor-400 by establishing a PCB-dechlorinating soil culture in liquid medium containing 0.5 mm glass beads. PCB-dechlorination activity in liquid cultures with glass beads appeared to depend on the size of the glass beads, and soil-free cultures with 0.05-, 1.0- or 2.0 mm glass beads did not dechlorinate PCBs. Soil-free culture without glass beads also failed to dechlorinate PCBs. The soil-free culture containing 0.5 mm glass beads dechlorinated 42.6 ± 12.0 mol% in total PCBs. This soil-free culture was more effective than soil culture for dechlorinating PCBs ranging from dichlorinated PCBs to tetrachlorinated PCBs. Clone analysis of the 16S rRNA gene sequences showed that one of the predominant groups of microorganisms in the soil-free culture comprised heat-tolerant and spore-forming bacteria from the phylum Firmicutes. Heat treatment (100 °C, 10 min) did not destroy the PCB-dechlorination activity of the soil-free culture with glass beads. These results suggest that unknown species of the phylum Firmicutes were involved in PCB dechlorination in the soil-free culture. In this study, we succeeded in using a liquid medium containing glass beads as an inorganic soil substitute and showed that such a medium enhances PCB-dechlorination activity. Our study provides valuable information for developing PCB-bioremediation techniques using dechlorinating bacteria in anoxic contaminated soils and sediments.