Versatile Hybrid Nanoplatforms for Treating Periodontitis with Chemical/Photothermal Therapy and Reactive Oxygen Species Scavenging
Versatile Hybrid Nanoplatforms for Treating Periodontitis with Chemical/Photothermal Therapy and Reactive Oxygen Species Scavenging
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DOI：
10.1016/j.cej.2023.142293
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发表时间：
2023-03
影响因子：
15.1
通讯作者：
Pei Wang;Liang Wang;Yilan Zhan;Yifan Liu;Ziqiang Chen;Jia Xu;Jun Guo;Jun-Ren Luo;Junchao Wei;Fei Tong;Zhihua Li
中科院分区：
文献类型：
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作者：
Pei Wang;Liang Wang;Yilan Zhan;Yifan Liu;Ziqiang Chen;Jia Xu;Jun Guo;Jun-Ren Luo;Junchao Wei;Fei Tong;Zhihua Li
由于牙周炎的临床刮宫术的局限性，彻底清除深层牙周袋的牙菌斑和牙周组织修复需要在适当的药物治疗的帮助下进行改进。尽管用于辅助牙周炎治疗的各种局部给药系统（LDDS）取得了显著的发展，但仍存在一些问题，例如相对较低的抗生物膜效果和由过量的活性氧（ROS）引起的组织损伤。为了解决这些问题，我们通过简单而有效的多巴胺（PP NPs）自聚合在单分散普鲁士蓝纳米颗粒（PB NPs）表面包覆聚多巴胺（PDA），然后负载米诺环素（Mino）来制备通用纳米平台（PPM NPs）。值得注意的是，这些集成的基于纳米复合物的PB NP作为光热剂增强了菌斑生物膜在NIR激光照射下的抗菌光热疗法（PTT）中的玻璃化治疗效果。此外，所设计的纳米复合材料有效地清除了氧化应激Raw267.4细胞和人牙周膜细胞的ROS，这归因于PB纳米酶的酶样活性和PDA中邻苯二酚的还原。体外和体内结果表明，牙周炎中生物膜的死染色和炎性因子的蛋白质印迹结果与游离Mino溶液的比较。我们制造的基于纳米平台的PB NPs方便地用PDA修饰，不仅通过组合疗法显著提高了抗菌效果，而且还利用纳米酶的酶样活性有效地清除细胞ROS，从而有助于牙周炎的治疗。
Given the limitations of clinical curettage for periodontitis, the thorough removal of dental plaque at deep periodontal pockets and periodontal tissue repair require improvement with the assistance of appropriate medical therapy. Despite the significant developments of various local drug delivery systems (LDDSs) for adjuvant periodontitis therapy, several issues exist, such as the relatively low anti-biofilm effect and the tissue damage caused by excessive reactive oxygen species (ROS). To address the issues, we fabricated the versatile nanoplatforms (PPM NPs)viacoating polydopamine (PDA) on the surface of monodispersed Prussian blue nanoparticles (PB NPs) through the facile yet efficient self-polymerization of dopamine (PP NPs), and then loading minocycline (Mino). Notedly, these integrative nanocomposites-based PB NPs as photothermal agents enhanced the therapeutic effect of plaque biofilmsin vitroutilizing the antibacterial photothermal therapy (PTT) under irradiation of NIR laser. Further, the designed nanocomposites efficiently scavenged ROS over oxidative-stress Raw267.4 cells and human periodontal ligament cells, attributing to the enzyme-like activity of PB nanozymes and the reducibility of catechol in PDA.In vitroandin vivoconsequences demonstrate the significantly augmented antibacterial and anti-inflammatory effects from the live/dead staining of biofilms and western blot results of inflammatory factors compared with free Mino solution in periodontitis. Our fabricated nanoplatforms-based PB NPs conveniently modified with PDA not only significantly improved the antibacterial effect via the combinational therapies but also efficiently scavenged cellular ROS utilizing the enzyme-like activity of nanozymes contributing to the treatment of periodontitis.