High Serum Trypsin Levels and the-409 T/T Genotype of PRSS1 Gene Are Susceptible to Neonatal Sepsis
High Serum Trypsin Levels and the-409 T/T Genotype of PRSS1 Gene Are Susceptible to Neonatal Sepsis
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高血清胰蛋白酶水平和 PRSS1 基因的-409 T/T 基因型易患新生儿败血症
DOI：
10.1007/s10753-014-9904-3
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发表时间：
2014-10-01
期刊：
影响因子：
5.1
通讯作者：
Chen, Hong
中科院分区：
文献类型：
--
作者：
Chen, Qingquan;Xue, Heng;Chen, Hong
新生儿败血症仍然是新生儿发病和死亡的重要且常见的原因，特别是在发展中国家。本研究的目的是确定血清胰蛋白酶水平和阳离子胰蛋白酶原（PRSS1）基因的基因型是否可以作为预测新生儿败血症的标志物。检测50例新生儿期感染婴儿和56例健康对照新生儿的血清胰蛋白酶水平和PRSS1基因型。感染婴儿进一步分为脓毒症组婴儿（n = 18）和非脓毒症感染婴儿组（n = 32）。通过直接测序分析PRSS1的基因型，并通过免疫分析法测定血清胰蛋白酶水平。结果表明，感染婴儿血清胰蛋白酶中值（31.90 ng/mL）显着高于对照组（12.85 ng/mL；P = 0.030）。更重要的是，脓毒症亚组（50.95 ng/mL）的血清胰蛋白酶中位数显着高于无脓毒症亚组（19.10 ng/mL）和对照组（12.85 ng/mL）的感染婴儿（分别为 P = 0.015 和 P = 0.002）。此外，与具有 C/T 基因型的婴儿 (12.80 ng/mL；P = 0.005) 相比，具有 PRSS1 T/T (37.90 ng/mL) 基因型的婴儿的中位血清胰蛋白酶水平显着较高。这项研究表明，血清胰蛋白酶和 PRSS1 的 rs10273639 C/T 被揭示是预测新生儿败血症的新标志物。
Neonatal sepsis remains an important and common cause of morbidity and mortality among newborn infants, especially in developing countries. The aim of the present study was to determine whether serum trypsin levels and genotypes of cationic trypsinogen (PRSS1) gene could be served as markers for predicting neonatal sepsis. The serum trypsin levels and genotypes of PRSS1 were examined in both 50 infants with infection during neonatal period and 56 healthy neonates as controls. The infected infants were further subdivided into infants with sepsis group (n = 18) and infected infants without sepsis (n = 32). The genotype of PRSS1 was analyzed by direct sequencing, and the serum trypsin level was measured by immunoassay. It showed that the median value of serum trypsin was significantly higher in infected infants (31.90 ng/mL) than in controls (12.85 ng/mL; P = 0.030). More importantly, sepsis subgroup (50.95 ng/mL) had significantly higher median serum trypsin than infected infants without sepsis subgroup (19.10 ng/mL) and controls (12.85 ng/mL) (P = 0.015 and P = 0.002, respectively). Additionally, the median serum trypsin levels were found significantly higher in infants who had T/T (37.90 ng/mL) genotype of PRSS1 compared with those who had C/T genotype (12.80 ng/mL; P = 0.005). This study suggested that serum trypsin and rs10273639 C/T of PRSS1 were revealed to be novel markers for predicting neonatal sepsis.