B cells malignantly transformed by human immunodeficiency virus are polyclonal.
B cells malignantly transformed by human immunodeficiency virus are polyclonal.
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人类免疫缺陷病毒恶性转化的B细胞是多克隆的。
DOI：
10.1006/viro.1998.9445
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Astrin,SM
中科院分区：
文献类型：
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作者：
Rodriguez-Alfageme,C;Chen,Z;Sonoda,G;Testa,JR;Damiani,RD;Astrin,SM
人类免疫缺陷病毒（HIV）和EB病毒（EBV）在获得性免疫缺陷综合征（AIDS）相关淋巴瘤发生中的作用尚不清楚。人类B细胞系（B-HIV），用于研究艾滋病相关的淋巴瘤发生，含有EB病毒和HIV基因组，并被恶性转化。该细胞系通过将来自EBV血清阳性供体的B细胞暴露于HIV而产生。为了研究HIV感染时发生的独立转化事件的数量，我们试图确定该细胞系中存在多少转化谱系。经荧光原位杂交证实，B-HIV有多个不同的HIV整合位点（≥25个）。作为对照，我们分析了HIV感染的人T细胞8 E5的克隆细胞系，发现HIV序列仅位于染色体13的q22带。我们的结论是，B-HIV是多克隆的，病毒序列位于不同的B-HIV细胞中的多个可变染色体位点。
The roles that human immunodeficiency virus (HIV) and Epstein–Barr virus (EBV) play in the genesis of aquired immune deficiency syndrome (AIDS)-related lymphomas are not understood. A human B cell line (B-HIV), developed to study AIDS-related lymphomagenesis, contains EBV and HIV genomes and is malignantly transformed. This line was produced by exposing B cells from an EBV-seropositive donor to HIV. To investigate the number of independent transformation events that took place at the time of HIV infection, we sought to determine how many transformed lineages are present in this cell line. B-HIV was found to have multiple different sites of HIV integration (≥25) as determined by fluorescencein situhybridization. As a control, we analyzed a clonal cell line of HIV-infected human T cells, 8E5, and found HIV sequences located exclusively at band q22 in chromosome 13. We conclude that B-HIV is polyclonal, and viral sequences are located at multiple variable chromosomal sites in different B-HIV cells.