Expression of SCC-S2, an antiapoptotic molecule, correlates with enhanced proliferation and tumorigenicity of MDA-MB 435 cells
Expression of SCC-S2, an antiapoptotic molecule, correlates with enhanced proliferation and tumorigenicity of MDA-MB 435 cells
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DOI：
10.1038/sj.onc.1207123
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发表时间：
2004-01-15
期刊：
影响因子：
8
通讯作者：
Kasid, U
中科院分区：
文献类型：
--
作者：
Kumar, D;Gokhale, P;Kasid, U
SCC-S2/GG2-1/NDED是最近发现的一种由转录因子NF-kappaB激活诱导的抗凋亡分子。在这里，我们研究了SCC-S2在体外和体内细胞生长调节中的作用。使用抗肽抗体的Western blotting发现，内源性SCC-S2与人乳腺癌细胞（MDA-MB 231）和肾癌细胞（RCC-RS）中的21kda细胞质蛋白相似。免疫荧光检测方法显示，在COS-1转染物中，flag标记的人SCC-S2在细胞质中定位。与对照载体转染相比，稳定转染了flag标记的SCC-S2 cDNA的MDA-MB 435人癌细胞表现出更高的生长速度，通过细胞活力测量(>为2倍；n=3； P
SCC-S2/GG2-1/NDED is a recently discovered antiapoptotic molecule induced by the activation of the transcription factor NF-kappaB. Here we have examined a role of SCC-S2 in cell growth regulation in vitro and in vivo. Western blotting using an antipeptide antibody revealed endogenous SCC-S2 as a similar to21kDa cytosolic protein in human breast cancer cells (MDA-MB 231) and renal carcinoma cells (RCC-RS). The immunofluorescence detection method showed the cytosolic localization of FLAG-tagged human SCC-S2 in COS-1 transfectants. MDA-MB 435 human cancer cells stably transfected with the FLAG-tagged SCC-S2 cDNA exhibited increased growth rate as compared to control vector transfectants, as measured by the cell viability (>twofold; n=3; P