The agony of choice: how to find a suitable CPP for cargo delivery
The agony of choice: how to find a suitable CPP for cargo delivery
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选择之苦：如何找到合适的CPP进行货物运输
DOI：
10.1002/psc.2396
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发表时间：
2012
影响因子：
2.1
通讯作者：
Boisguerin P
中科院分区：
文献类型：
--
作者：
Müller J;Triebus J;Kretzschmar I;Volkmer R;Boisguerin P
成功和有效的细胞递送仍然是医学领域的主要障碍。细胞穿透肽(CPPs)的使用已成为包括药物在内的广泛分子内化的最重要工具之一。选择一种CPP用于一种生物应用仍然是困难的，因为没有普遍存在的CPP满足不同的要求。在我们的案例中，我们正在寻找一种合适的CPP来通过简单的共孵育策略传递促凋亡KLA多肽(KLAKLAKLAKLAK)。为此，我们选择了三种不同的细胞系(成纤维细胞、癌细胞和巨噬细胞)，并研究了六种不同的CPP单独以及与KLA多肽混合后的摄取和亚细胞定位。此外，我们使用带有羧胺或羧化C-末端的CPP，并分析了C-末端对内化和货物运输的影响。我们可以清楚地表明，细胞对CPP的摄取不仅取决于所用的CPP和细胞系，而且受到其C末端的化学性质(摄取：羧胺基CPPS >； 羧化CPP)的高度影响，并能影响其细胞定位。我们成功地在三个细胞系中传递了KLA多肽，并了解到在这里，C末端对于有效的多肽传递是至关重要的。最后，我们用酰胺化的MPG多肽 ： Kla与非洲绿猴肾成纤维细胞(Cos-7)的混合物诱导小鼠白血病单核细胞巨噬细胞(RAW 264.7)和人乳腺癌细胞(MCF7)的凋亡。 ： Kla羧化整合素多肽。版权所有©2012欧洲多肽协会和John Wiley&Sons，Ltd.
Successful and effective cellular delivery remains a main obstacles in the medical field. The use of cell‐penetrating peptides (CPPs) has become one of the most important tools for the internalisation of a wide range of molecules including pharmaceuticals. It is still difficult to choose one CPP for one biological application because there is no ubiquitous CPP meeting the diverse requirements. In our case, we are looking for a suitable CPP to deliver the pro‐apoptotic KLA peptide (KLAKLAKKLAKLAK) by a simple co‐incubation strategy. For that reason, we selected three different cell lines (fibroblastic, cancerous and macrophagic cells) and studied the uptake and subcellular localisation of six different CPPs alone as well as mixed with the KLA peptide. Furthermore, we used the CPPs with a carboxyamidated or a carboxylated C‐terminus and analysed the impact of the C‐termini on internalisation and cargo delivery. We could clearly showed that the cellular CPP uptake is not only dependent on the used CPP and cell line but also highly affected by its chemical nature of the C‐terminus (uptake: carboxyamidated CPPs > carboxylated CPPs) and can influence its cellular localisation. We successfully delivered the KLA peptide in the three cell lines and learned that here as well, the C‐terminus is crucial for an effective peptide delivery. Finally, we induced apoptosis in mouse leukaemic monocyte macrophage (RAW 264.7) and in human breast adenocarcinoma (MCF‐7) cells using the mixture of amidated MPG peptide : KLA and in african green monkey kidney fibroblast (Cos‐7) cells using carboxylated integrin peptide : KLA. Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd.
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影响因子：
11.2
作者：
Standley SM;Toft DJ;Cheng H;Soukasene S;Chen J;Raja SM;Band V;Band H;Cryns VL;Stupp SI
通讯作者：
Stupp SI
强效放射性标记的特异性标记改变了细胞穿透肽的摄取
影响因子：
2.9
作者：
C. Walther;I. Ott;R. Gust;I. Neundorf
通讯作者：
I. Neundorf
DOI：
10.1073/pnas.97.24.13003
发表时间：
2000-11-21
影响因子：
11.1
作者：
Wender, PA;Mitchell, DJ;Rothbard, JB
通讯作者：
Rothbard, JB
影响因子：
10.8
作者：
Watkins, Catherine L.;Brennan, Paul;Jones, Arwyn T.
通讯作者：
Jones, Arwyn T.
影响因子：
3.9
作者：
Lemeshko, Victor V.
通讯作者：
Lemeshko, Victor V.