Genotypic and phenotypic characterization of human immunodeficiency virus type 1 isolates circulating in pregnant women from Mozambique
Genotypic and phenotypic characterization of human immunodeficiency virus type 1 isolates circulating in pregnant women from Mozambique
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DOI：
10.1007/s00705-008-0215-6
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发表时间：
2008-11-01
影响因子：
2.7
通讯作者：
Brindeiro, Rodrigo de Moraes
中科院分区：
文献类型：
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作者：
Abreu, Celina Monteiro;Brindeiro, Patricia A.;Brindeiro, Rodrigo de Moraes
这项工作评估了来自不同地理区域的HIV-1亚型和来自莫桑比克的未服用药物的HIV阳性孕妇的表型数据。我们分析了莫桑比克三个地区75个患者的POL序列和亚型分布，发现大多数样本与C亚型聚在一起。在北部地区，在总共28个样本中，发现5个(类似于18%)A亚型，3个(类似于11%)亚型D和2个(类似于7.1%)马赛克(A/C/D和C/D)，而18个(64.3%)分离株为C亚型。在南方地区，20份标本中只有1株(5%)分离株为D亚型，其余19株(95%)分离株为C亚型。所有27株(100%)分离株均来自中部C分支，未发现对IP、NNRTI或NRTI的原发耐药性突变。在测试的任何菌株中都没有表型耐药性的证据，这表明无论是蛋白酶的多态性，还是RT基因第215密码子的多态性都不会导致表型耐药性的增加。这一发现表明，世卫组织指示的HAART方案很可能在莫桑比克取得成功。
This work evaluated HIV-1 subtypes from different geographic regions and phenotypic data from drug-naive HIV-positive pregnant women from Mozambique. We analyzed 75 pol sequences from patients and the distribution of the subtypes in three regions of Mozambique and found that the majority of samples analyzed clustered with subtype C. In the northern region, multiple variants were found 5 (similar to 18%) subtype A, 3 (similar to 11%) subtype D and 2 (similar to 7.1%) mosaics (A/C/D and C/D), whereas 18 (64.3%) isolates were subtype C, from a total of 28 samples. Already in the southern region, only one (5%) isolate of 20 samples was subtype D, and the other 19 (95%) isolates were subtype C. All 27 (100%) isolates from the central region grouped within clade C. No primary resistance mutations to IP, NNRTI or NRTI were found. There was no evidence of phenotypic resistance in any of the isolates tested, suggesting that neither the polymorphism in the protease, nor the one found at codon 215 of the RT gene caused an increase in phenotypic resistance. This finding suggests that HAART regimens indicated by WHO will probably be successful in Mozambique.