The evaluation of putative virulence factors of Helicobacter pylori for gastroduodenal disease by use of a short-term Mongolian gerbil infection model
The evaluation of putative virulence factors of Helicobacter pylori for gastroduodenal disease by use of a short-term Mongolian gerbil infection model
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DOI：
10.1086/338772
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发表时间：
2002-02-01
影响因子：
6.4
通讯作者：
Omata, M
中科院分区：
文献类型：
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作者：
Akanuma, M;Maeda, S;Omata, M
幽门螺杆菌的毒力决定因素很少在体内进行测试。我们进行了这项研究，以建立一个动物模型，为他们的筛选。将6周龄雄性蒙古沙土鼠接种野生型幽门螺杆菌(Tn2)及其缺失CAg总致病岛(Tn2DeltakagE)、HP0499(Tn2DeltaHP499)和HP0638(Tn2DeltaHP638)的等位基因突变株(n=5)。3周后处死动物，比较胃部细菌密度和炎症程度。除接种Tn2DeltaHP638的动物外，其余动物均有感染。TN2和TN2DeltaHP499，而不是TN2DcagE和TN2Deltakag PAI，虽然细菌密度相似，但引起了强烈的炎症。蒙古沙鼠模型可用于体内毒力决定因素的筛选，这证实了CAG PAI的重要性，但对HP0499的重要性提出了质疑。
Few virulence determinants of Helicobacter pylori have been tested in vivo. We conducted this study to establish an animal model for their screening. Six-week-old male Mongolian gerbils were inoculated with wild-type H. pylori (TN2) or its isogenic mutant with deletion of cagE (TN2DeltacagE), total cag pathogenicity island (TN2Deltacag PAI), HP0499 (TN2DeltaHP499), or HP0638 (TN2DeltaHP638) (n = 5 each). The animals were killed 3 weeks later, and the density of bacteria and the degree of inflammation in the stomach were compared. Infection was established in all animals except those inoculated with TN2DeltaHP638. TN2 and TN2DeltaHP499, but not TN2DcagE and TN2Deltacag PAI, induced intense inflammation, although the densities of bacteria were similar. The Mongolian gerbil model was useful for the screening of virulence determinants in vivo, which confirmed the importance of cag PAI while questioning that of HP0499.