DNA methylation in eukaryotes: kinetics of demethylation and de novo methylation during the life cycle.
DNA methylation in eukaryotes: kinetics of demethylation and de novo methylation during the life cycle.
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DOI：
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发表时间：
1990-02
期刊：
影响因子：
3.3
通讯作者：
Sarah P. Otto;Virginia Walbot
中科院分区：
文献类型：
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作者：
Sarah P. Otto;Virginia Walbot
我们提出了一个真核DNA甲基化和去甲基化的动力学模型；该模型包含了从头甲基化的值和维持甲基化的错误率。从这些方程中可以得出一个平衡，即在一个细胞世代中，新甲基化的位置比例等于去甲基化的位置比例。这一平衡被经验地确定为维持甲基化的水平。然后，我们以玉米和小鼠为模型物种，为参数选择了合理的值。一般来说，如果基因组高甲基化或低甲基化，随着时间的推移，它将逐渐接近维持甲基化的平衡水平；每个生命周期只发生一次抑制甲基化的事件可以保持相对低甲基化状态。虽然所开发的方程式在这里用作评估整个基因组事件的框架，但它们也可用于评估特定位置随时间的甲基化和去甲基化的速率。
We present a model for the kinetics of methylation and demethylation of eukaryotic DNA; the model incorporates values for de novo methylation and the error rate of maintenance methylation. From the equations, an equilibrium is reached such that the proportion of sites which are newly methylated equals the proportion of sites which become demethylated in a cell generation. This equilibrium is empirically determined as the level of maintenance methylation. We then chose reasonable values for the parameters using maize and mice as model species. In general, if the genome is either hypermethylated or hypomethylated it will approach the equilibrium level of maintenance methylation asymptotically over time; events occurring just once per life cycle to suppress methylation can maintain a relatively hypomethylated state. Although the equations developed are used here as framework for evaluating events in the whole genome, they can also be used to evaluate the rates of methylation and demethylation in specific sites over time.