A general method for phasing novel complex RNA crystal structures without heavy-atom derivatives.
A general method for phasing novel complex RNA crystal structures without heavy-atom derivatives.
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一种无需重原子衍生物的新型复杂 RNA 晶体结构定相的通用方法。
DOI：
10.1107/s0907444908011578
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Scott,WilliamG
中科院分区：
文献类型：
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作者：
Robertson,MichaelP;Scott,WilliamG
The crystallographic phase problem [Muirhead & Perutz (1963), Nature (London), 199, 633–638] remains the single major impediment to obtaining a three-dimensional structure of a macromolecule once suitable crystals have been obtained. Recently, it was found that it was possible to solve the structure of a 142-nucleotide L1 ligase ribozyme heterodimer that possesses no noncrystallographic symmetry without heavy-atom derivatives, anomalous scattering atoms or other modifications and without a model of the tertiary structure of the ribozyme [Robertson & Scott (2007), Science, 315, 1549–1553]. Using idealized known RNA secondary-structural fragments such as A-form helices and GNRA tetraloops in an iterative molecular-replacement procedure, it was possible to obtain an estimated phase set that, when subjected to solvent flattening, yielded an interpretable electron-density map with minimized model bias, allowing the tertiary structure of the ribozyme to be solved. This approach has also proven successful with other ribozymes, structured RNAs and RNA–protein complexes.
