The draft genome sequence of Scheffersomyces segobiensis strain DSM 27193, a yeast capable of producing palmitoleic acid-rich lipids
The draft genome sequence of Scheffersomyces segobiensis strain DSM 27193, a yeast capable of producing palmitoleic acid-rich lipids
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DOI：
10.1007/s13205-021-03040-y
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发表时间：
2021-11
期刊：
影响因子：
2.8
通讯作者：
Xinhai Zhou;Lijie Xu;D. Zhou;Jie Zhou;F. Xin;Wenming Zhang;Xiujuan Qian;W. Dong;M. Jiang;K. Ochsenreither
中科院分区：
文献类型：
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作者：
Xinhai Zhou;Lijie Xu;D. Zhou;Jie Zhou;F. Xin;Wenming Zhang;Xiujuan Qian;W. Dong;M. Jiang;K. Ochsenreither
在这项研究中，对最近分离的产油酵母Scheffersomyces segobiensisDSM 27193的基因组草案进行了测序和分析，该酵母可以积累高含量的棕榈油酸（POA）。迄今为止，只有很少的研究报道了 Scheffersomyces segobiensis 生产富含 POA 的脂质。 ITS区域分析表明菌株DSM 27193与Pichia segobiensis和Scheffersomyces stipitis密切相关。菌株 DSM 27193 组装后的基因组草图大小为 14.8 Mb，包含 5477 个编码蛋白序列，G + C 含量为 40.83%。在注释的基因中，鉴定出由ole1和ole2编码的两个硬脂酰辅酶A去饱和酶，它们可能参与POA积累。进一步分析S中富含POA的脂质合成途径基因。 segobiensisDSM 27193 将为通过代谢工程人工合成 POA 提供更多见解。
In this study, the draft genome of the recently isolated oleaginous yeastScheffersomyces segobiensisDSM 27193, which can accumulate high content of palmitoleic acid (POA), was sequenced and analyzed. Only few studies have reported about POA-rich lipid production byScheffersomyces segobiensisso far. The ITS region analysis indicated that strain DSM 27193 is closely related toPichia segobiensisandScheffersomyces stipitis. The size of the assembled draft genome of strain DSM 27193 is 14.8 Mb, containing 5477 encoded protein sequences with a G + C content of 40.83%. Among the annotated genes, two stearoyl-CoA desaturases encoded byole1andole2were identified which are potentially involved in POA accumulation. Further analysis of POA-rich lipid synthesis pathway genes inS. segobiensisDSM 27193 will provide additional insights for POA artificial synthesis through metabolic engineering.