A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes
A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes
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DOI：
10.1038/12902
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发表时间：
1999-09-01
影响因子：
46.9
通讯作者：
van Bel, AJE
中科院分区：
文献类型：
--
作者：
Knoblauch, M;Hibberd, JM;van Bel, AJE
一加仑膨胀飞针器使飞针器能够将样品注入原核生物和真核生物的亚细胞区室。该方法利用玻璃注射器内的galinstan（一种镓、铟和锡的液态金属合金）的热致膨胀，通过直径约0.1 μ m的尖端排出样品。与传统的毛细血管相比，galinstan的狭窄尖端造成的损伤更小，热致膨胀可以很好地控制注射速度。我们演示了将路西法黄和路西法黄葡聚糖缀合物注射到蓝藻中，以及注射到高等生物的细胞核和叶绿体中。将含有bla基因的质粒注射到蓝藻中，产生了对氨苄西丁耐药的转化培养。将含有绿色荧光蛋白基因的DNA注入烟草和蚕豆的叶绿体后，在附着叶片中表达绿色荧光蛋白。
A galinstan expansion femtosyringe enables femtoliter to attoliter samples to be introduced into prokaryotes and subcellular compartments of eukaryotes. The method uses heat-induced expansion of galinstan (a liquid metal alloy of gallium, indium, and tin) within a glass syringe to expel samples through a tip diameter of about 0.1 mu m. The narrow tip inflicts less damage than conventional capillaries, and the heat-induced expansion of the galinstan allows fine control over the rate of injection. We demonstrate injection of Lucifer Yellow and Lucifer Yellow-dextran conjugates into cyanobacteria, and into nuclei and chloroplasts of higher organisms. Injection of a plasmid containing the bla gene into the cyanobacterium Phormidium laminosum resulted in transformed ampiciltin-resistant cultures. Green fluorescent protein was expressed in attached leaves of tobacco and Vicia faba following injection of DNA cantaining its gene into individual chloroplasts.