The effect of diclofenac sodium on neural tube development in the early stage of chick embryos
The effect of diclofenac sodium on neural tube development in the early stage of chick embryos
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DOI：
10.5603/fm.a2018.0080
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发表时间：
2019-01-01
影响因子：
1.2
通讯作者：
Albay, S.
中科院分区：
文献类型：
--
作者：
Ertekin, T.;Bilir, A.;Albay, S.
背景：神经管畸形是一种先天性的中枢神经系统畸形。遗传易感性和一些环境因素在神经管畸形的发生发展中起着重要作用。本研究旨在探讨双氯芬酸钠对哺乳动物脊椎发育第一个月的鸡胚模型中神经管发育的影响。材料和方法：将75枚无特定病原体的受精卵孵化28h，分成5组，每组15枚。在这个阶段，双氯芬酸钠是通过胚层下途径给药的。孵化至48h结束，打开所有卵子，从胚膜中剥离胚胎，进行形态学和组织病理学评价。结果：随着双氯芬酸钠剂量的增加，早期鸡胚中线闭合出现缺陷。两组间神经管位置(打开或关闭)的差异有统计学意义。此外，与对照组相比，高剂量实验组的冠臀长度、体节数显著减少。结论：双氯芬酸钠对鸡胚神经元的发育有影响。双氯芬酸钠的确切致畸机制尚不清楚，应对其进行研究。
Background: Neural tube defects are congenital malformations of the central nervous system. Genetic predisposition and some environmental factors play an important role in the development of neural tube defects. This study aimed to investigate the effects of diclofenac sodium on the neural tube development in a chick embryo model that corresponds to the first month of vertebral development in mammals.Materials and methods: Seventy-five fertile, specific pathogen-free eggs were incubated for 28 h and were divided into five groups of 15 eggs each. Diclofenac sodium was administered via the sub-blastodermic route at this stage. Incubation was continued till the end of the 48th h. All eggs were then opened and embryos were dissected from embryonic membranes and evaluated morphologically and histopathologically.Results: It was determined that the use of increasing doses of diclofenac sodium led to defects of midline closure in early chicken embryos. There were statistically significant differences in neural tube positions (open or close) among the groups. In addition; crown-rump length, somite number were significantly decreased in high dose experimental groups compared with control group.Conclusions: This study showed that development of neurons is affected in chicken embryos after administration of diclofenac sodium. The exact teratogenic mechanism of diclofenac sodium is not clear; therefore it should be investigated.