Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET
Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET
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DOI：
10.1021/ja108626w
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发表时间：
2011-02-09
影响因子：
15
通讯作者：
Magennis, Steven W.
中科院分区：
文献类型：
--
作者：
Sabir, Tara;Schroeder, Gunnar F.;Magennis, Steven W.
分支DNA结构在DNA复制、修复和重组中起着关键作用，除了是DNA纳米技术的关键构建块之外。在这里，我们结合联合收割机单分子多参数荧光检测和分子动力学模拟，给出了一个通用的方法来确定全局结构的分支DNA在溶液中。我们揭示了一个开放的，平面结构的叉状DNA分子与三个双链臂，并证明了离子诱导的构象变化。该结构将作为DNA-蛋白质相互作用研究的基准。
Branched DNA structures play critical roles in DNA replication, repair, and recombination in addition to being key building blocks for DNA nanotechnology. Here we combine single-molecule multiparameter fluorescence detection and molecular dynamics simulations to give a general approach to global structure determination of branched DNA in solution. We reveal an open, planar structure of a forked DNA molecule with three duplex arms and demonstrate an ion-induced conformational change. This structure will serve as a benchmark for DNA-protein interaction studies.